Eukaryotic transfer RNA (tRNA) precursors undergo sequential processing steps to become mature tRNAs. In humans, ELAC2 carries out 3′ end processing of both nucleus-encoded (nu-tRNAs) and mitochondria-encoded (mt-tRNAs) tRNAs. ELAC2 is self-sufficient for processing of nu-tRNAs but requires ...
et al. RNase P without RNA: identification and functional reconstitution of the human mitochondrial tRNA processing enzyme. Cell 135, 462–474 (2008). This Article describes the first report of the unique proteinaceous RNase P complex found in human mitochondria, which is devoid of a catalytic ...
Overall, mtDNA mutations located in the transcribed regions were also found in RNA-seq with similar VAFs, except for a fraction of transfer RNA (tRNA) mutations showing much higher VAFs in transcripts due to the accumulation of unprocessed tRNA precursors during the processing of polycistronic ...
RNase P without RNA: Identification and functional reconstitution of the human mitochondrial tRNA processing enzyme Cell, 135 (2008), pp. 462-474 View PDFView articleView in ScopusGoogle Scholar 14 T. Suzuki, Y. Yashiro, I. Kikuchi, Y. Ishigami, H. Saito, I. Matsuzawa, S. Okada, M. Mi...
For instance, mitochondria use fMet-tRNA as an initiator of protein synthesis. Mammalian mitochondrial ribosomes have small 28S and large 39S subunits, forming a 55S protein complex, which is active in mitochondria and functions to translate mitochondrial mRNAs encoded in mtDNA (Collatz et al., ...
Transcription of human mtDNA is driven from within the noncoding region by promoters on both strands to form polycistronic transcription units (Gustafsson et al.2016). Processing of these units is driven in part by the natural positioning and folding of mt-tRNA structures, which are excised by...
Ojala D, Montoya J, Attardi G: tRNA punctuation model of RNA processing in human mitochondria. Nature. 1981, 290: 470-474. 10.1038/290470a0. Article CAS PubMed Google Scholar Boore JL, Daehler LL, Brown WM: Complete sequence, gene arrangement, and genetic code of mitochondrial DNA of the...
However, there are also direct junctions between two PCGs (atp8/atp6, atp6/cox3, nad6/cob and nad4L/nad4) where other cleavage signals, different from tRNA gene secondary structures, may be involved in the processing of the polycistronic primary transcript [23]. In this respect, hairpin...
The trm1 mutation of Saccharomyces cerevisiae is a single nuclear mutation that affects a specific base modification of both cytoplasmic and mitochondrial tRNA. Transfer RNA isolated from trm1 cells lacks the modified base N2,N2-dimethyl... SR Ellis,MJ Morales,JM Li,... - 《Journal of Biologic...
The tRNA is translocated across mitochondrial membranes in its aminoacylated form and remains relatively stable inside the organelle. Possible roles of amino... NSEA B,IAK B,RPM A,... - 《Febs Letters》 被引量: 46发表: 1996年 The Mitochondrial Protein Import Pathway: Are Precursors Imported ...