Inhibition of internalization had no effect on TrkB kinase, but inhibition of TrkB kinase prevents the modulation of TrkB internalization, suggesting a critical role of the tyrosine kinase in the activity-dependent receptor endocytosis. These results demonstrate an activity- and Ca2+-dependent modulation...
Receptor tyrosine kinases (RTK) belong to a major class of cell surface receptors that transduce extracellular signals to elicit diverse intracellular responses. Upon binding to specific ligand, the RTKs become dimerized and autophosphorylated at tyrosine residues. This creates binding sites to recruit ...
Here we describe targeting of TrkB-binding peptide-conjugated liposomes (PCL) to the TrkB-expressing mouse macrophage-like cell line RAW264, and to all-trans-retinoic acid-treated neuron-like TrkB⁺ SH-SY5Y human neuroblastoma cells.Binding and internalization of PCL was monitored by flo...
First, AP-2 is associated and co-trafficked with autophagosomes (Fig. 1) containing active TrkB receptors post-internalization (Fig. 5). Second, and consistent with this, we find AP-2 to be largely dispensable for BDNF-induced TrkB endocytosis in cultured neurons (see Supplementary Fig. 5n–...
Current therapies that target the B-cell receptor pathway or the inhibition of anti-apoptotic proteins do not prevent the progressive forms of chronic lymphocytic leukemia (CLL), have low long-term efficacy and are subject to therapeutic resistance. Deciphering the mechanisms of leukemic cell survival...
Motoneuron degenerationALSWhile Brain-derived Neurotrophic Factor (BDNF) has long been implicated in treating neurological diseases, recombinant BDNF protein has failed in multiple clinical trials. In addition to its unstable and adhesive nature, BDNF can activate p75NTR, a receptor mediating cellular ...
While Brain-derived Neurotrophic Factor (BDNF) has long been implicated in treating neurological diseases, recombinant BDNF protein has failed in multiple clinical trials. In addition to its unstable and adhesive nature, BDNF can activate p75(NTR), a receptor mediating cellular functions opposite to ...
In the context of recent hypotheses regarding the function of truncated trk B receptors, sequence analysis indicates that trk B.T4 may bear putative signaling/internalization sequences.doi:10.1385/JMN:17:1:81Farzin ForooghianLjubomir KojicQiang Gu...