The protein has\nspecificity and exolytic activity for the peptidoglycan cell\nwall of untreated, live Staphylococcus aureus from many growth phases i e\nstationary, logarithmic and biofilm growth. The recombinant\ntriple fusion protein comprising the three functional antimicrobial domains is\ndesigned ...
Unfolding of the protein to obtain complete exchange should be performed only if the subsequent refolding results in complete regeneration of the functional protein structure (33). For the purposes of the following discussion, 1HN spins are assumed to be fully present at all amide sites. 9.1.2 ...
ILF2 (red), and HNRNPK (cyan), along with nuclear staining using DAPI (blue). scale bar: 10μm (G) Schematic representation of HNRNPK with functional protein domains. HNRNPK had been truncated within regions: 1-143aa, 1-213aa, 144-213aa, 214-463aa, and 144-463aa.H-IRelative enri...
The partially refolded protein was then used as a model system for testing proteolytic enzymes as probes for quantitative assay of fully aligned triple-helical molecules. Pepsin and chymotrypsin both digested fully denatured procollagen. However, digestion times of greater than 60 min were required, ...
Despite radiotherapy ability to significantly improve treatment outcomes and survival in triple-negative breast cancer (TNBC) patients, acquired resistance to radiotherapy poses a serious clinical challenge. Protein disulfide isomerase exists in endoplas
1E, F). These data indicated a potential role for NRBP1 in promoting TNBC progression and highlighted this protein for further functional and mechanistic interrogation. Fig. 1: NRBP1 and its expression in breast cancer. A Schematic representation of NRBP1 structure. Abbreviations: ES-rich, ...
Heat shock protein 90 (Hsp90) is a chaperone protein frequently expressed in breast cancer [116] that stabilizes the structural and functional integrity of many oncogenic clients and can act as a protective “biochemical buffer” [117]. Onalespib (AT13387) is an inhibitor that targets the N-...
Mitogen-activated protein kinase PNPLA8: Patatin-like phospholipase domain-containing lipase 8 PA: Phosphatidic acid PC: Phosphatidylcholine PE: Phosphatidylethanolamine PG: Phosphatidylglycerol PI: Phosphatidylinositol PI3K: Phosphatidylinositol-3-kinase PL: Phospholipids PLA1: Phospholipase A1 ...
N-propeptidecleavageC-propeptidecleavageN-terminusCollagenstructuraldomainC-terminus N-propeptidecleavageC-propeptidecleavage N-terminusCollagenstructuraldomainC-terminus FigureSource:KuivaniemiH,TrompG.TypeIIIcollagen (COL3A1):Geneandproteinstructure,tissuedistribution,andassociateddiseases.Gene.2019Jul30;707:151-...
To confirm whether the chimeric protein on the surface of OMVs maintains its affinity towards EGFR, a Cell-ELISA assay was conducted against EGFR overexpressing cells, namely HT-29, HCT-116, A-549, 4T1, and negative control, HEK-293T (Fig. 3). The binding characteristics of scFv-OMVs wer...