Structural characterization of the full length protein with its non-lectin part (115 of 250 residues total) will help understand the multi functionality of this potent cellular effector. Here, we report 1H, 13C, and 15N chemical shift assignments as determined by heteronuclear NMR spectroscopy ....
To delineate the domain in DDA3 responsible for EB1-binding, a GST-EB1 pull-down assay was accomplished with 293T cell extracts containing various deletion mutants of DDA3 separately. Full-length GFP-DDA3 and its two C-terminal deletion mutants, GFP- DDA3122-363 and GFP-DDA3238-363, ...
(c) Schematic drawing of EB1 deletion mutants. Residue numbers at domain boundaries are indicated. CH, calponin homology domain; CC, coiled-coil region. (d) DDA3 interacted with the C-terminal domain of EB1. Purified GST-EB1 deletion mutants were used to isolate GFP-DDA3 from 293T cell...
KEYTRUDA, as a single agent, is indicated for the treatment of patients with metastatic NSCLC whose tumors express PD-L1 (TPS ≥1%) as determined by an FDA-approved test, with disease progression on or after platin...
Eisai’s Corporate Concept is “to give first thought to patients and people in the daily living domain, and to increase the benefits that health care provides.” Under this Concept [also known as our human healt...
(Thermo) and segmented to create a binary image stack containing fatty tissue (subcutaneous fat and IMAT). Morphological operations (object closing/filling and image subtraction) were then performed to isolate subcutaneous adipose, IMAT, and skeletal muscle in the thigh. Tissue volumes were then ...
In order to fully capture the main aspects of an ocean energy system, the present digital representation framework has accounted for: Elements of the technology design (physical domain), phases of the technology lifecycle (process domain) and constraints from the context (external environment) A ...
(Figure2) are aberrant and possible on the cell surface. These interactions were also accompanied with higher levels of the protein expression, such as β-catenin and VEGF (Figure1), whereas higher protein expression augments possibility to produce abnormal proteins. Complement also plays the ...
Sorting nexin 3 (SNX3) belongs to a sub-family of sorting nexins that primarily contain a single Phox homology domain capable of binding phosphoinositides and membranes. We report the complete 1H, 13C and 15N resonance assignments of the full-length human SNX3 protein and identification of its...
Eisai’s Corporate Concept is “to give first thought to patients and people in the daily living domain, and to increase the benefits that health care provides.” Under this Concept [also known as our human...