62. Wu, B., et al.,Structural insights into the specific recognition of DSR by the YTH domain containing protein Mmi1.Biochem Biophys Res Commun, 2017.491(2): p. 310-316. 63. Alarcon, C.R., et al.,HNRNPA2B1 Is a Mediator of m(6)A-Dependent Nuclear RNA Processing Events.Cell, 20...
62. Wu, B., et al.,Structural insights into the specific recognition of DSR by the YTH domain containing protein Mmi1.Biochem Biophys Res Commun, 2017.491(2): p. 310-316. 63. Alarcon, C.R., et al.,HNRNPA2B1 Is a Mediator of m(6)A-Dependent Nuclear RNA Processing Events.Cell, 20...
(3)] is restricted to the phagocytic cup, promoting phagocytosis. The PtdIns(3,4,5)P(3) 5-phosphatase (5-ptase) Src homology 2 (SH2) domain-containing inositol-5-phosphatase 1 (SHIP1) inhibits phagocytosis. We report here that another PtdIns(3,4,5)P(3)-5-ptase, the 72-kDa-5-...
1f). When Tea2 and Tip1:GFP were added to the PEG-containing assay, we observed both motor traces at the lattice and bright comets at microtubule plus-ends (Fig. 1g). These plus-end-bound comets could transfer from the end of one microtubule to the lattice of another, spread out, be...
Structural characterization of the full length protein with its non-lectin part (115 of 250 residues total) will help understand the multi functionality of this potent cellular effector. Here, we report 1H, 13C, and 15N chemical shift assignments as determined by heteronuclear NMR spectroscopy ....
1.Inoculate a single colony from a freshly streaked plate into 5 ml of LB medium containing the appropriate antibiotic for the plasmid and host strain. 2.Incubate with shaking at 200 rpm at 37℃ overnight. 3.Inoculate 50 ml of LB medium containing the appropriate antibiotic with 0.5 ml of...
An example of one such CAP-Gly domain-containing +TIP is p150glued: the largest out of eleven subunits of the dynactin complex. Dynactin is essential to nearly all functions of dynein, the most prominent minus-end directed motor protein [74]. Dynein plays a particularly important role in axon...
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because ...
Find additional tips, troubleshooting help, and resources within ourProtein Purification and Isolation Support Center. 引用和文献(3) 搜索 引用和文献 Abstract The hCds1 (Chk2)-FHA domain is essential for a chain of phosphorylation events on hCds1 that is induced by ionizing radiation. ...
The antibody also contains mutations in the constant domain of the heavy chain that reduces binding to complement protein C1q and to Fcγ receptors to avoid complement- and antibody-mediated cytotoxicity. In this trial (NCT02489448), we tested the hypothesis that durvalumab administered concurrently ...