Galectin-3, an adhesion/growth regulatory lectin, has a unique trimodular design consisting of the canonical carbohydrate recognition domain, a collagen-like tandem-repeat section, and an N-terminal peptide with two sites for Ser phosphorylation. Structural characterization of the full length protein ...
To delineate the domain in DDA3 responsible for EB1-binding, a GST-EB1 pull-down assay was accomplished with 293T cell extracts containing various deletion mutants of DDA3 separately. Full-length GFP-DDA3 and its two C-terminal deletion mutants, GFP- DDA3122-363 and GFP-DDA3238-363, ...
(c) Schematic drawing of EB1 deletion mutants. Residue numbers at domain boundaries are indicated. CH, calponin homology domain; CC, coiled-coil region. (d) DDA3 interacted with the C-terminal domain of EB1. Purified GST-EB1 deletion mutants were used to isolate GFP-DDA3 from 293T cell...
KEYTRUDA, as a single agent, is indicated for the treatment of patients with metastatic NSCLC whose tumors express PD-L1 (TPS ≥1%) as determined by an FDA-approved test, with disease progression on or after platinu...
Eisai’s Corporate Concept is “to give first thought to patients and people in the daily living domain, and to increase the benefits that health care provides.” Under this Concept [also known as our human healt...
Cultured cells were lysed with lysis buffer from Cell Signaling Technology, and lysates were cleared at 3000 rpm and 4°C or treated ARPE was lysed with a buffer containing a low concentration of detergent for detection of β-catenin (Figure1D). The buffer and the procedure for preparation of...
(A) Schematic drawing of a female hop cone that is composed of a central spine: the strig, bracteoles, bracts, and the characteristic lupulin glands that are tiny yellow sacs containing the hop oils. (B) Chemical structure of xanthohumol (3′-[3,3-dimethylallyl]-2′,4′,4-trihydroxy-6...
the improvements in thigh composition did not translate to improvements in function. A retrospective analysis identified an association where those who were Vitamin D3insufficient did not improve muscle function after an amino acid cocktail containing HMB while those who were Vitamin D3sufficient increase...
In order to fully capture the main aspects of an ocean energy system, the present digital representation framework has accounted for: Elements of the technology design (physical domain), phases of the technology lifecycle (process domain) and constraints from the context (external environment) A ...
Sorting nexin 3 (SNX3) belongs to a sub-family of sorting nexins that primarily contain a single Phox homology domain capable of binding phosphoinositides and membranes. We report the complete 1H, 13C and 15N resonance assignments of the full-length human SNX3 protein and identification of its...