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Protein quality control, U-box-containing E3 ubiquitin ligases join the fold. Trends Biochem Sci 27, 368–375. Deb S, Sankaranarayanan S, Wewala G, Widdup E, Samuel MA (2014). The S-domain receptor kinase At- ARK2 and the U-box/ARM-repeat- containing E3 ubiquitin 732 植物学报 51(5...
CRISPR/Cas9-mediated double-nucleotide deletion in the OsPUB67 target sequence led to a truncated OsPUB67 protein at the U-box domain. C and D: Phenotypes of OsPUB67 OE3, WT, and M1 plants under control and drought stress conditions. E: The survival rates of the OsPUB67 OE3, WT, ...
2e–g). A firefly luciferase complementation imaging assay based on transient expression34 suggested ABI1 may interact with either the whole PUB12 or PUB13 protein (Fig. 2h) and with the ARM domain (Fig. 2i). The interaction of FLS2 with the PUB13 ARM domain was used as a positive ...
Human E4B, also called UFD2a, is a U box-containing protein that functions as an E3 ubiquitin ligase and an E4 polyubiquitin chain elongation factor. E4B is thought to participate in the proteasomal degradation of misfolded or damaged proteins through association with chaperones. The U box ...
Protein quality control: U-box-containing E3 ubiquitin ligases join the fold. Trends in biochemical sciences 27, 368–375 (2002). Article CAS PubMed Google Scholar Borden, K. L. & Freemont, P. S. The RING finger domain: a recent example of a sequence—structure family. Current opinion ...
Previously, we first identified U-box Protein 1 (CaPUB1) in hot pepper (Capsicum annuum L. cv Pukang). The U-BOX E3 ligase encodes a protein containing a single U-box motif in its N-terminal region and the activity of CaPUB1 inversely correlated with drought-tolerance. Notably, CaPUB1 ...
Abstract:U— boxdomainwasahighconservedmotifexistingwidelyineukaryotes,especiallyinplants.edo mainofU—box wassimilartothatoftheRING—finger.BothU—boxmotifandRING— fingercouldfunctionasubiquitin—ligaseE3tocatalyzethe targetproteinsubiquitinateddegradation.TheU— boxmotifalsoWasthemainfunctionalfactorofubiquitin...
The 632-amino acid residue Arabidopsis AtPUB14 protein (8) was selected for structure studies, because it contains a typical Arabidopsis U-box domain (Fig. 1b) and has a domain architecture consisting of a UND domain (16) followed by a U-box domain and several ARM repeats (Fig. 1a). ...
1) U-box domain U-box结构域 1. To investigate the function and correlation of CHIP wit h its possible substrates proteins, we constructed the eukaryotic expression vec tor containing CHIP full-length coding sequence and its mutants with either TPR orU-box domainmutation and its deletion withU-...