TRI Reagent Solution Ambion_100 ml100 ml价格电询原厂型号 AM9738 雷琪货号 RH147502 品牌 单件重量 1kg 货期 45天 单位: 瓶 包装: 瓶 库存: 0 数量: 瓶 + - 1瓶起订,增量1瓶 此商品不支持在线订购,请联系电话:400-080-8817累计成交0笔0条评价 商品信息 商品参数 评价(0) TRI Reagent ...
3-chloropropane to the TRI Reagent in Sample Preparation, step 2. After precipitation and multiple washes, the DNA is dissolved in 8 mM NaOH. The solution is neutralized and the DNA is ready for analysis. The resulting DNA is suitable for PCR, restriction enzyme digestion, and Southern ...
2.試劑準備a.RNA純化 均質化:TRIReagent 層析:BCP或chloroform 沈澱與清洗:100%isopropanol,75%ethanol(用DEPC水配製) 回溶:FORMAzol,或0.5%SDS或DEPCwaterb.DNA純化 純化:100%Ethanol 清洗:0.1Mtrisodiumcitratein10%ethanol,75%ethanol 回溶:8mMNaOHc.蛋白質純化 純化:Acetone 清洗:washsolution(0.3Mguanidine...
Using TRI Reagent® solution TRI Reagent® starting material can be either fresh or frozen tissue, or cell samples of almost any size—the protocol is easily scalable. This RNA isolation method is well-recognized and referenced in numerous publications, a testament to its reliability. ...
TRI Reagent combines phenol and guanidine thiocyanate in a mono-phase solution to facilitate the immediate and most effective inhibition of RNase activity. A biological sample is homogenized or lysed in TRI Reagent and the homogenate is separated into aqueous and organic phases by (cat. no. BP 15...
蛋白質純化 純化:Acetone 清洗:wash solution (0.3M guanidine hydrochloride in 95% ethanol + 2.5% glycerol) 回溶:1%SDS 或 10M urea 或其他適用試劑 Pan Asia Biomedical Technology Inc. 亞醫醫學器材股份有限公司 .tw - 1 - Molecular Research Center TRI reagent (TR-118) Ver: 111ED RNA 純化操作...
TRIzol (or TRI Reagent) is a monophasic solution of phenol and guanidinium isothiocyanate that simultaneously solubilizes biological material and denatures protein. After solubilization, the addition of chloroform causes phase separation (much like extraction with phenol:chloroform:isoamyl alcohol), where ...
DNA wash solution: 0.1 M trisodium citrate in 10% ethanol (no pH adjustment required), 2–3 ml per 1 ml of TRI Reagent used in the initial homogenization: 75% ethanol, 1.5–2 ml per 1 ml TRI Reagent used in the initial homogenization 8 ...
Solution:Unexpectedly low RNA yield can occur when the initial solubilization step is not effective; e.g., it is too short or not enough TRIzol has been used. Make sure that the solution is clearand of uniform viscosity. INTRODUCTION TRIzol solubilization and extraction is a relatively recently...
Haematologic Technologies,Haematologic Technologies Inc,Hyglos,IBL,immunodx,Immunostep,ImmunoVision,innovagen,iris-biotech,jackson抗体,lifecore,LSBIO,macrocyclics,medkoo biosciences,NEB,NIBSC,NIST标准品,omega试剂盒,Percipio Biosciences,PhosphoSolution,promocell,sigma,thermo,TLC标准品,tocris,试剂盒,细胞株,重组...