品牌: sigma 货号: T9424-200ML 是否进口: 是 产品名称:TRI试剂® RNA 分离试剂 英文名称:TRI Reagent® 产品规格:For processing tissues, cells cultured in monolayer or cell pellets 注意:Sigma现货||T9424-200ML|TRI试剂® RNA 分离试剂,TRI Reagent® For processing tissues, cells cultured...
Total RNA was extracted by using TRI-reagent (Sigma, PT). cDNA was generated using NZY First-Strand cDNA Synthesis Kit (NZYTech, PT). Real Time PCR was performed on a BioRad CFX96 quantitative real time PCR machine using the SYBR Green JumpStart master mix (Sigma, PT). The primer sequen...
1.定义组成 2.Return and Value function 3.马尔科夫决策过程(MDP) 1.定义组成 2.Policy 3.马尔科夫决策过程的价值函数 3.Bellman Expectation Equation immediate reward: 概述 state: Agent在环境中采取action后,在环境中新观测到的状态。 reward: Agent在环境中采取action后,环境反馈的奖励信号。奖励具有延时性,...
Total RNA was extracted by using TRI-reagent (Sigma, PT). cDNA was generated using NZY First-Strand cDNA Synthesis Kit (NZYTech, PT). Real Time PCR was performed on a BioRad CFX96 quantitative real time PCR machine using the SYBR Green JumpStart master mix (Sigma, PT). The primer ...
Boron Trifluoride - Butanol Reagent (10-20%)[for Esterification](1mL 10) (10-20%)[用于酯化](1mL×10) 价格: 价格电议 产品详情:中文名称:三氟化硼-丁醇试剂中文别名:三氟化硼-正丁醇CAS No:692-39-7EINECS号:231-569-5分子式:C4H10BF3O安全说明:RTECSED2275... Boron Trifluoride - Isopropyl ...
Transfection of HEK293T cells for immunoprecipitation was performed by the HighGene plus transfection reagent (ABclonal, China). To establish stable gene expression cell lines, α-catenin-VN173-IRES-VC155-vinculin overexpressing plasmids and shRNA plasmids were transfected into HEK293T cells together ...
recycling ribosome collision ribosome profiling Introduction One mechanism the cell can use to monitor the quality of protein and mRNA is to sense the state of thetranslatingribosome. A stalled translation cycle could indicate an mRNA defect and directly lead to synthesis of aberrant proteins or a ...
The cells were maintained with MEM (Sigma-Aldrich) supplemented with 10% dialyzed fetal bovine serum (FBS; Invitrogen, Carlsbad, CA, USA). The medium contained 100 U/ml penicillin, 100 μg/ml streptomycin and 50 μg/ml amphotericin-B. Sawano cells were plated on 35- and 60-mm ...
All solvents and reagents were reagent grade; H2O was distilled and deionised. Tanshinone IIA was purchased from Sigma (St. Louis, MO) and was dissolved in ethanol (100%). The two turmeric preparations contained the curcuminoids curcumin, desmethoxycurcumin, and bis-demethoxycurcumin as follows:...
. MCF-7 cells were transfected using lipofectamine Plus reagent from the Life Technologies, Inc. Cells were collected and lysates were prepared according to the manufacturer's protocol for luciferase assay (Promega Corp., Madison, Wis.). Antibodies for p53, p21WAF1/CIP1, Mdm2, Protein ...