高效:相对于传统硫氰酸胍/氯化铯方法,TRI reagent®产量更高。从新鲜组织或细胞开始的完整RNA提取流程可在一小时内完成。 可轻松扩展的RNA分离 可适用于多种来源:人、植物、酵母、细菌或病毒 相对于传统的硫氰酸胍/氯化铯具有更高的产量 法律信息 TRI Reagent is a registered trademark of Molecular Research Center,...
MolecularResearchCenterTRIreagent(TR-118).mrcgeneVer:111EDPanAsiaBiomedicalTechnologyInc.亞醫醫學器材股份有限公司.pan-asia-bio.com.tw-1-TRIreagentRNA/DNA/蛋白質三效分離試劑中文操作說明(RNA純化)基本原理:TRIreagent是一種新型totalRNA抽取試劑,內含異硫氰酸胍等物質,能迅速破碎細胞,抑制細胞釋放出的...
DNA wash solution: 0.1 M trisodium citrate in 10% ethanol (no pH adjustment required), 2–3 ml per 1 ml of TRI Reagent used in the initial homogenization: 75% ethanol, 1.5–2 ml per 1 ml TRI Reagent used in the initial homogenization...
TRIreagentRNADNA中文操作說明(RNA.pdf,Molecular Research Center TRI reagent (TR-118) Ver: 111ED TRI reagent RNA / DNA / 蛋白質 三效分離試劑 中文操作說明 (RNA 純化) 基本原理: TRI reagent 是一種新型 total RNA 抽取試劑,內含異硫氰酸胍等物質,能迅速破碎細胞
TRI Reagent LS 是一种快速、方便的试剂,用于同时分离人、动物、植物、酵母、细菌和病毒来源的液体样本中的 RNA、DNA 和蛋白质。 方便的单步液相分离可同时分离 RNA、DNA 和蛋白质。该程序是 Chomczynski 和 Sacchi 报道的用于总 RNA 分离的单步方法的改良版,可快速有效地处理液体样本。TRI Reagent LS 在样品量...
Chomczyrtski et al, (3) reported recently that a new reagent, TR[ Reagent~(TM) (Molecular Research Center, Cincinnati, OH. USA), was suitable for use in isolating RNA, DNA and protein from a single culture. We explored the use of this reagent for thestudy of metabolism of ...
Total RNA was extracted from multiple excised lacrimal glands using TRI-reagent (Molecular Research Center, Cincinnati, OH), and quantified using a NanoDrop 2000 (Thermo Scientific). For the reverse transcriptase assay, 2 μg RNA was converted to cDNA using a QuantiTect Reverse Transcription Kit...
TRIzol (Invitrogen) or TRI Reagent (Molecular Research Center, Inc.) TRIzol LS (for liquid samples or yeast and bacteria; see Step 1.v) Equipment Centrifuge (for large-scale isolations) Dry ice Alternatively, liquid nitrogen can be used with a precooled mortar and pestle to prepare the tiss...
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Total RNA was isolated using TRIzol reagent (Thermo Fisher Scientific), according to the manufacturer’s protocol. RT-PCR was performed as described previously65. The primers LP023805 and RP023805 (Supplementary Table 2) were used to detect SIZ1 expression. The primers as described in the pre...