DNA is precipitated from the phenol phase and interphase of samples that have been homogenized (or lysed) in 1 ml of TRI Reagent (step 5 in the RNA Isolation Protocol). After a series of washes to remove residual phenol, the DNA pellet is ...
TRI Reagent® Protocol 常见问题解答 (FAQ) One to ten nanograms of total RNA per 15 µL RT reaction is recommended for use with the TaqMan MicroRNA Assay. We have successfully detected miRNAs in as little as 25 pg of total RNA (~100 cells) in samples where the miRNA was highly abun...
TRI Reagent® starting material can be either fresh or frozen tissue, or cell samples of almost any size—the protocol is easily scalable. This RNA isolation method is well-recognized and referenced in numerous publications, a testament to its reliability. ...
Following the provided the standard protocol, we typically obtain crude RNA yields of 4 to 5 mg/mL from co-transcriptional capping with CleanCap technology. Are CleanCap analogs available in GMP grade? Yes, please inquire about them from the SKU table provided above or email sales@trilinkbiotech...
This protocol is suitable for the preparation of KATs containing pyridines, esters, nitro groups, and halides.doi:10.1002/anie.201403931Gábor ErsLaboratorium für Organische Chemie, Department of Chemistry and Applied Bioscience, ETH Zürich, 8093 Zürich (Switzerland) http://www.bode.ethz.chYo ...
The operationally simple protocol is quite fast and does not require the external halogenation source at 110 degrees C in 20-60 minutes and furnished halogenated fused heterocycles 4 a-p, 5 a-k, 6 a-l, 10 a-i, 11 a, 12 a-b in up to 96% yields. The gram-scale synthesis, wide ...
Phenanthridines are a core structure of many naturally occurring bioactive alkaloids.11 (B) Xu and colleagues have reported a flexible protocol involving a two-step strategy to assemble 11H-indolo[3,2-c]quinoline starting from acyclic alkyne substrates. Hendrickson reagent is used as the second ...
The operationally simple protocol is quite fast and does not require the external halogenation source at 110°C in 20–60 minutes and furnished halogenated fused heterocycles 4a–p, 5a–k, 6a–l, 10a–i, 11a, 12a–b in up to 96% yields. The gram-scale synthesis, wide substrate scope, ...
Application of di(benzotriazol-1-yl)methanimine reagent; Purification protocol for preparing the compounds; Sensitivity of the amine condensation to electronic and steric effects; Use of oxidizers in the synthesis of tri- and tetrasubstituted derivatives.Katritzky...
A novel protocol for the synthesis of potassium acyltrifluoroborates from aryl- and heteroarylhalides using zwitterionic S-ethyl thioformamide trifluoroboronate as CO- and BF 3 -source is presented.doi:10.1002/chin.201502175Gabor ErosDep. Chem. Angew. Biowiss., ETH-Zuerich, CH-8093 Zuerich, ...