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TRI Reagent® Protocol 常见问题解答 (FAQ) One to ten nanograms of total RNA per 15 µL RT reaction is recommended for use with the TaqMan MicroRNA Assay. We have successfully detected miRNAs in as little as 25 pg of total RNA (~100 cells) in samples where the miRNA was highly abun...
Simple Modifications to Standard TRIzol® Protocol Allow High-Yield RNA Extraction from Cells on Resorbable Materials RNA ExtractionTRIzolAcid Guanidium Thiocyanate - Phenol - Chloroform ExtractionTRI ReagentTRIsureResorbable bioceramics are attractive for medical applications such... J Tsz,L Yan,WH Tsang...
TRI Reagent® starting material can be either fresh or frozen tissue, or cell samples of almost any size—the protocol is easily scalable. This RNA isolation method is well-recognized and referenced in numerous publications, a testament to its reliability. ...
Protocol overview Resources PublicationsDESCRIPTION The FavorPrep™ After Tri-Reagent RNA Clean-Up Kit is designed for fast cleaning up RNA which was isolated by other methods, such as guanidine isothiocyanate/phenol/chloroform extraction or lithium chloride/phenol/chloroform extraction. It is also suit...
A novel protocol for the synthesis of potassium acyltrifluoroborates from aryl- and heteroarylhalides using zwitterionic S-ethyl thioformamide trifluoroboronate as CO- and BF3-source is presented.GaborDep.ErosDep.YoDep.KushidaDep.JeffreyDep.
An Efficient, Eco-Friendly, One-Pot Protocol for the Synthesis of 2-Oxazolines Promoted by Ionic Liquid/Indium Chloride 2-Oxazolines have been synthesized using a solventless ionic liquid melt in good yields at ambient temperatures. The efficiency of various Lewis acid catal... R Kamakshi,BSR ...
Following the provided the standard protocol, we typically obtain crude RNA yields of 4 to 5 mg/mL from co-transcriptional capping with CleanCap technology. Yes, please inquire about them from the SKU table provided above or emailsales@trilinkbiotech.com. ...
A novel protocol for the synthesis of potassium acyltrifluoroborates from aryl- and heteroarylhalides using zwitterionic S-ethyl thioformamide trifluoroboronate as CO- and BF3-source is presented.doi:10.1002/chin.201502175Gabor ErosDep. Chem. Angew. Biowiss.ETH-ZuerichYo Kushida...
DNA is precipitated from the phenol phase and interphase of samples that have been homogenized (or lysed) in 1 ml of TRI Reagent (step 5 in the RNA Isolation Protocol). After a series of washes to remove residual phenol, the DNA pellet i...