A novel TREX1 inhibitor, VB-85680, upregulates cellular interferon responsesSECOND messengers (Biochemistry)T cellsGENETIC regulationCELL linesINTERFERONSActivation of the cGAS-STING pathway plays a key role in the innate immune response to cancer through Type-1 Interferon (IFN...
(a) exposure to DNA damaging agents, including IR, hydroxyurea [29], DNA topoisomerase I inhibitor camptothecin [98], and UVC exposure [84,85]; (b) nuclear envelope rupture due to mechanical pressure inside the crowded solid tumor [99] (c) granzyme A-mediated cell death [80]. The ...
We found that treatment of Trex1-deficient mice with the reverse transcriptase (RT) inhibitor AZT did not rescue from lethality (Figures S2). However, the mouse genome contains hundreds of diverse RT enzymes, only a fraction of which is susceptible to inhibition by AZT, and it is unclear ...
observed in response to 8GyX3. Analysis of tumour-infiltrating DCs (TIDCs) revealed a marked increase in CD8α+TIDCs within TSA tumours after 8GyX3 but not 20 Gy radiation, consistent with the requirement for IFNβ for recruitment to the tumour of CD8α+DCs, essential for optimal priming of...
weusedTrex1gene-deficientmicetostudytherelationshipbetweencelastrolandDNA-inducedinterferonregulatorypathways.HereweshowthatcelastrolisapotentinhibitorwhichleadstoinhibitionoftheTBK1-IRF3activationandthedownstreamIFNresponsetriggeredbycytosolicnucleicacids,asshownbyinvivoandinvitrotests.Moreover,celastroltreatmentmarkedly...
from TSA, 4T1 or MDA-MB-231 parental cells and their derivatives were extracted in RIPA buffer (Sigma, Cat # R0278-50 ml) containing protease inhibitor cocktail (Sigma, Cat # 11836153001). Proteinconcentrations were determined with BCA method (Thermo Scientific, Cat # 23227). A totalof ...
We describe, to our knowledge for the first time, the efficacy of the JAK1/2 inhibitor ruxolitinib in a patient with TREX1-related skin disease. Parental consent was obtained for the use of ruxolitinib on a compassionate basis. 展开
[4]Salojin C, Gardberg A, Vivat V, et al. 765 The first-in-class small molecule TREX1 inhibitor CPI-381 demonstrates type I IFN induction and sensitization of tumors to immune checkpoint blockade[J]. Journal for ImmunoTherapy of Cancer, 2021, 9(Suppl 2): A800. ...
SFE10058 Human inhibitor of apoptosis,IAP ELISA KIT 人细胞凋亡抑制因子(IAP)ELISA定性/定量试剂盒KIT SFE10059 Human colony-stimulating factor,CSFE ELISA KIT 人集落刺激因子(CSFE)ELISA定性/定量试剂盒KIT SFE10060 Human monocyte interferon gamma inducing factor,MIGF ELISA KIT 人γ干扰素诱导单核细胞因子...
All lysis buffers were supplemented with 1 mm PMSF and one Complete Mini protease inhibitor mixture tablet (Roche Applied Science) prior to use. Lysates used as input controls were normalized for protein content and separated by SDS-PAGE using 4–12% gradient SDS-polyacrylamide gels (Invitrogen)....