[4]Salojin C, Gardberg A, Vivat V, et al. 765 The first-in-class small molecule TREX1 inhibitor CPI-381 demonstrates type I IFN induction and sensitization of tumors to immune checkpoint blockade[J]. Journal for ImmunoTherapy of Cancer, 2021, 9(Suppl 2): A800. [5]Francica B, Burdett...
Here, we characterize the activity of VB-85680, a potent small-molecule inhibitor of TREX1. We first demonstrate that VB-85680 inhibits TREX1 exonuclease activity in vitro in lysates from both human and mouse cell lines. We then show that treatment of intact cells w...
We found that treatment of Trex1-deficient mice with the reverse transcriptase (RT) inhibitor AZT did not rescue from lethality (Figures S2). However, the mouse genome contains hundreds of diverse RT enzymes, only a fraction of which is susceptible to inhibition by AZT, and it is unclear ...
This TREX1 inhibitor has been recently validated in cell lysates and cell-based assays to inhibit human TREX1 [55,57]. We are looking forward to seeing if such a compound would have an effect on cancer pre-clinical models. From a drug development perspective, these TREX1 inhibitors should ...
Trypsin inhibitor 胰蛋白酶抑制剂 CAS:9035-81-8 在线交易 48小时发货 少货必赔 破损包赔 百克赛斯生物科技(镇江)有限公司 2年 查看详情 ￥1200.00/盒 上海 Cys-C 小鼠半胱氨酸蛋白酶抑制剂/胱抑素CELISA检测试剂盒 细胞生物学 帛科品牌 上海帛科生物技术有限公司 4年 查看详情 ￥800.00/盒 广东深圳 ...
Radiotherapy is under investigation for its ability to enhance responses to immunotherapy. However, the mechanisms by which radiation induces anti-tumour T cells remain unclear. We show that the DNA exonuclease Trex1 is induced by radiation doses above 12–18 Gy in different cancer cells, and att...
weusedTrex1gene-deficientmicetostudytherelationshipbetweencelastrolandDNA-inducedinterferonregulatorypathways.HereweshowthatcelastrolisapotentinhibitorwhichleadstoinhibitionoftheTBK1-IRF3activationandthedownstreamIFNresponsetriggeredbycytosolicnucleicacids,asshownbyinvivoandinvitrotests.Moreover,celastroltreatmentmarkedly...
Proteins from TSA, 4T1 or MDA-MB-231 parental cells and their derivatives were extracted in RIPA buffer (Sigma, Cat # R0278-50 ml) containing protease inhibitor cocktail (Sigma, Cat # 11836153001). Protein concentrations were determined with BCA method (Thermo Scientific, Cat # 23227). A ...
We describe, to our knowledge for the first time, the efficacy of the JAK1/2 inhibitor ruxolitinib in a patient with TREX1-related skin disease. Parental consent was obtained for the use of ruxolitinib on a compassionate basis. 展开
All lysis buffers were supplemented with 1 mm PMSF and one Complete Mini protease inhibitor mixture tablet (Roche Applied Science) prior to use. Lysates used as input controls were normalized for protein content and separated by SDS-PAGE using 4–12% gradient SDS-polyacrylamide gels (Invitrogen)....