Trap-seq基于Ribo-seq技术,通过利用核糖体作为“标记”来捕获并分离由核糖体翻译的mRNA。在Trap-seq实验中,首先通过转化目标细胞或组织,使其表达核糖体上特定的融合蛋白,这些蛋白与核糖体上的蛋白有一定亲和性,并且可以用亲和纯化技术进行高效分离。然后,用亲和纯化技术富集核糖体-融合蛋白- mRNAs复合物,并用RNase酶降...
TRAP-Seq Targeted Purification of Polysomal mRNA Targeted purification of polysomal mRNA (TRAP-Seq) maps translating mRNAs under various conditions(Jiao et al., 2010). In this method, tagged ribosomal proteins are expressed in cells. The tagged ribosomal proteins are purified and the RNA isolated....
该研究通过比较早期缺镁情况下基因转录水平(RNA-seq)和翻译水平(TRAP-seq)的差异,鉴定出一些仅在翻译水平响应镁供给变化的新基因。进一步结合遗传学筛选,发现一个碱性亮氨酸拉链b-ZIP家族转录因子HY5,两个钙离子转运体CAX1和CAX3,和一...
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Translating ribosome affinity purification (TRAP) followed by RNA sequencing technology (TRAP-Seq) for quantitative assessment of plant translatomes. In Plant Functional Genomics: Methods and Protocols; Alonso, J.M., Stepanova, A.N., Eds.; Springer: New York, NY, USA, 2015; pp. 185-207....
CapTrap-Seq: A platform-agnostic and quantitative approach for high-fidelity full-length RNA sequencing Silvia Carbonell-Sala1, Tamara Perteghella1,2, Julien Lagarde1,3, Hiromi Nishiyori4,5, Emilio Palumbo1, Carme Arnan1, Hazuki Takahashi4,5, Piero Carninci4,5, Barbara Uszczynska-Ratajczak1...
RPL19-TRAPKI-Seq 揭示抗肿瘤化合物 SBF-1 干扰氧化磷酸化 OSW-1 是一种具有强大细胞毒性的甾体皂苷,其类似物 SBF-1 抗肿瘤活性相似且更易合成,但作用机制不明。研究人员用 RPL19-TRAPKI-Seq 研究 SBF-1,发现它会影响氧化磷酸化途径中的蛋白质复合物 I/III,导致线粒体相关基因下调。进一步实验表明,SBF-1...
TRAP samples in males and females as was the ubiquitin protein ligase adapterArrdc4. We verified the lack of differential expression ofArddc4after sleep deprivation at the level of the transcriptome through qPCR experiments from the total RNA samples of the young males for which TRAP-Seq was ...
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