Escherichia coli plasmid vectors characterized in that they have a 5 min -terminal untranslated region (inclusive of the propoter region and Shine-Dargano sequence) of the Escherichia coli lipoprotein gene, which region is improved to thereby enable direct production of useful polypeptides in ...
TransforMax EPI300 Electrocompetent E. coli and TransforMax EPI300 Chemically Competent E. coli F or Research Use Only. Not for use in diagnostic procedures.TransforMax™ EPI300™ Electrocompetent E. coli and TransforMax™ EPI300™ Chemically Competent E. coli are part of the Epicentre™...
TransforMax EC100D Electrocompetent E. coli Perform plasmid rescue for transposition mutants created with the EZ-Tn5 Transposome Kits. View Phage Display Library Construction ER2738 Electrocompetent Cells Create large phage display libraries with high efficiency cells recommended for peptide display. Vie...
Ekspresi gen penyandi kolin dehidrogenase (bet A) Esherichia coli K-12 dalam saccharomyces cerevisiae The growth rate of the transfomed yeast under non-selective growth condition w s lower than that of the non-transformed yeast, indicating that the a recombinant plasmid imposed a physiological ...
This was carried out by exposing the mixture of plasmid pND968 and competent cells of E. coli HB101 on a difference level of electroporation voltage at 1500, 2000 and 2500 Volts. After electroporation, the cells were resuspended in a recovery medium and then poured into ampicilin-containing ...
The nontransformed calli of normal wheat were capable of differentiating into whole plants on the agar-solidified N6 medium supplemented with 3% sucrose, 100 mg/l CH, 0.2% activated carbon, 0.1 mg/l 2, 4D, Fig 6. Digestion assay of pLGV2103 plasmid apnrodbeelefrcatrgompehnotr.eLsiasnieds...
WT mouse MDR3 Pgp and also Pgp mutants bearing single mutations in the Walker B sequence signature of NBD1 (E552Q) and NBD2 (E1197Q) were constructed in the pHIL-D2 plasmid vector (Invitrogen) and overexpressed in the yeast Pichia pastoris as previously described (34, 36). The condition...
The resulting plasmid, named pRLG, carried resistance genes to tetracycline, kanamycin and ampicillin. pRLG was transformed into the host strain Escherichia coli XL10-gold (Stratagene). pRLG was then transferred from E. coli XL10-gold to Acinetobacter sp. BD413, A. faecalis IS-18R and R. ...
The cDNA-containing plasmid vector obtained in the above manner can be introduced into a host for transformation thereof. Typical as the host is Escherichia coli. However, the host is not limited to this but may be, for example, Bacillus subtilis or Saccharomyces cerevisiae. The host can be...
A plasmid into which the papA gene was inserted in the correct orientation to the promoter contained in pTrc101 was selected and named pTrc-papA. FIG. 2 shows the process of the above-mentioned plasmid construction. The Escherichia coli JM109 strain carrying pTrc-papA was cultured in an LB ...