Disclosed are novel circular DNA plasmids useful as vectors in recombinant methods to secure high levels of E.coli expression of exogenous genes. Plasmids of the invention comprise discrete DNA sequences opera-tive to: (1) confer upon the plasmid the capacity for autonomous replication in a host...
In this study, Nattokinase(NK) gene was amplified by PCR using Bacillus subtilis chromosomal DNA as template and cloned into expressed vector, PBV220. After being analyzed by restriction enzyme and PCR, the NK gene was expressed in E.coli and expression products were proved to have fibrinolytic...
Plasmid Mediated Resistance to Cephalosporin and Adhesion Properties in E.ColiAdhesionbiofilmphysicochemical propertiesresistance plasmidIntroduction: The objective of this study is to evaluate the relationship between biofilm formation, surface characteristics and the presence of plasmid conferring resistance to ...
To survey this presumed diversity and its implications for the lineage of resistance plasmids we examined the transferability, sizes and EcoR1 restriction fragment sizes of plasmids in both Escherichia coli isolated randomly from poultry raised by 16 growers as they were being processed through two ...
A process for producing plasmid DNA E. coli cells comprises a pre-culture and fed-batch process. The culture media of the batch phase and the culture medium added during the feeding phase are chemically defined. The culture medium of the feeding phase contains a growth-limiting substrate and ...
mcr-3.5 was cloned into E. coli DH5α. The strain belonged to ST410, a type with a global distribution. Two colistin-resistant genes, mcr-1.1 and mcr-3.5, a carbapenemase gene blaNDM-5, and a 16S methylase gene rmtB were identified on different plasmids of IncHI2(ST3)/IncN, IncP...
of acquired colonies21, the assembly mixtures were introduced intoE. colicells by electroporation. For evaluation of the assembly fidelity, we picked 10 colonies from each assembly and analyzed them by restriction digestion. As shown in Fig.3b, both the assembly fidelity and the number of ...
F plasmid DNA transfer (tra) gene expression in Escherichia coli is regulated by chromosome- and F-encoded gene products. To study the relationship among these regulatory factors, we constructed low-copy plasmids containing a Φ(traY′-'lacZ)hyb gene that couples β-galactosidase and Lac permease...
In spite of peristaltic movements in the gut, and of apparently even distribution of E. coli as single cells in the intestinal mucus, the intestinal environment displays transfer kinetics different from those expected of a mixed, liquid culture, but quite similar to those of a biofilm. 展开 ...
performance of PlasX using a score threshold of either >0.5 or >0.9.e, Coefficients of the 200 gene families with the highest PlasX coefficients and that are thus most important for identifying plasmids.f, Maximum-likelihood phylogenetic tree of genes that are in PF10609 as well as either ...