Isolation of Plasmid DNA from Escherichia coli: The Mini-PrepJeffrey M. BeckerGuy A. CaldwellEve Ann Zachgo
mutans specific muramidase, mutanolysin. The use of this enzyme has enabled constant detection of this plasmid from all strains with high reliability, confirming the findings of cariogenic plasmids and their uniform molecular size in all strains of Strep. mutans tested....
The PureLink HiPure Plasmid Purification Kits allow isolation of high yields of highly pure ssM13 (single-stranded M13) DNA from bacteria. The kits are designed to efficiently isolate plasmid DNA fromE. coliin 1.5-2.5 hours using anion-exchange columns without the use of...
The method by which plasmids can be isolated from yeast or any other cells depends upon; (a) the volume of culture involved, (b) the purity of the DNA which is ultimately required; i.e. the use to which it will eventually be put, (c) how quickly the plasmid is required, and (d)...
From a library of Escherichia coli DNA, cloned into the vector plasmid pUC-8, several recombinant plasmids were isolated, which bind to this antibody. Thus, E. coli contains sequences, which in ``natural'' negatively supercoiled DNA, adopt a left-handed Z-DNA-like conformation. 展开 ...
A method is provided for using a silanized silica matrix to isolate a target nucleic acids, such as plasmid DNA, fragments of DNA, chromosomal DNA, or RNA from contaminants, including proteins, lipids
The PureLink™ HiPure Plasmid Purification Kits allow isolation of high yields of highly pure cosmid DNA. The kits are designed to efficiently isolate plasmid DNA from E. coli in 1.5-2.5 hours using anion-exchange columns without the use of any organic solvents or cesium chloride (CsCl). The...
Plasmid DNA The ISOLATE II portfolio includes a kit for simple, rapid, efficient recovery of plasmid DNA from even low bacterial culture volumes, without the need for hazardous reagents such as phenol. Separate protocols are provided for the column-based purification of high-copy plasmids, low-cop...
However, SV40 DNA excised from the plasmid replicates as well as authentic viral DNA and is fully infectious. SV40 mutants with small deletions or base substitutions have been isolated by in vitro site-specific or random local mutagenesis of recombinant DNA followed by cloning in E. coli. Many...
18. The apparatus of claim 16, wherein P2 and/or P3 is provided in solid or immobilized form. 19. A method for isolating plasmid DNA from bacteria by alkaline lysis, the method comprising: a) providing a bacterial suspension comprising bacteria having plasmid DNA; b) adding a modified P2...