20 mg/ml proteinase K phenolchloroform (50:50) isopropanol 70% ethanol 3M sodium acetate pH 5.2 Phase Lock GelTM (5 Prime, 3 Prime, Inc) 1.Grow E. coli culture overnight in rich broth. Transfer 1.5ml to a micro centrifuge tube and spin 2min. Decant the supernatant. Repeat with anothe...
Plasmid DNA isolation (i.e., plasmid preps, mini-preps, rapid DNA preps, among other procedures) remains a repetitious and tedious laboratory task. Plasmid DNA isolation from bacteria has traditionally been performed using the “alkaline lysis” method, in which bacteria are resuspended in lysis ...
Transfection-grade plasmid isolation kits are specifically designed for scientists who need to extract plasmid DNA (pDNA) with minimal levels of endotoxins from bacterial cultures. During the plasmid purification process, large amounts of endotoxins are released from lysed bacteria, which ...
Transfection-grade plasmid isolation kits are specifically designed for scientists who need to extract plasmid DNA (pDNA) with minimal levels of endotoxins from bacterial cultures. During the plasmid purification process, large amounts of endotoxins are released from lysed bacteria, which can neg...
Somkuti GA, Steinberg DH (1986) General method for plasmid DNA isolation from thermophilic lactic acid bacteria. J Biotechnol 3: 323-332.Somkuti, G.A. and Steinberg, D.H. (1986) General method for plasmid DNA isolation from thermo- philic lactic acid bacteria. J. Biotechnol. 3, 323-332...
HeLa Cells seeded in a 6-well plate were transfected with either 2 or 4 µg of pCI-neo®+GFP plasmid isolated from 100 ml of bacteria culture using the ZymoPURE Midiprep Kit, ZymoPURE Maxiprep Kit, or MN Midiprep Kit. GFP expressin was assessed 48 hours later in cell lysates using...
aDistribution of parameter values obtained using Bayesian inference to estimate growth kinetic parameters from OD measurements obtained for each strain in isolation. Diamonds representKlebsiellaspp. strains and circlesE. coliclones; filled symbols denote plasmid-bearing strains and empty symbols plasmid-free...
Isolation of cryptic plasmids from moderately halophilic eubacteria of the genus Halomonas. Characterization of a small plasmid from H. elongata and its us... Isolation of cryptic plasmids from moderately halophilic eubacteria of the genus Halomonas. Characterization of a small plasmid from H. ...
(HindII) in 1970. In 1978, a well-deserved Nobel Prize in Physiology or Medicine was awarded to Daniel Nathans, Werner Arber, and Hamilton Smith for their work on the discovery and isolation of DNA restriction and modification enzymes. Enzymes from a particular group of restriction endonucleases...
Plasmid DNA isolation 来自 百度文库 喜欢 0 阅读量: 4 申请(专利)号: US11603855 申请日期: 20061121 公开/公告号: US07867751B2 公开/公告日期: 20110111 申请(专利权)人: Xiyu Jia 发明人: X Jia 国省代号: US 摘要: The invention provides apparatus, reagents, and methods for rapidly ...