doi:10.1007/BF00367126C RohdeWorld J Microbiol BiotechnolWorld Journal of Microbiology & BiotechnologyRhode C (1995). Technical information sheet No. 12: plasmid isolation from bacteria: some fast procedures. World J Microbiol Biotechnol, 11(3): 367–369...
Plasmid DNA isolation (i.e., plasmid preps, mini-preps, rapid DNA preps, among other procedures) remains a repetitious and tedious laboratory task. Plasmid DNA isolation from bacteria has traditionally been performed using the “alkaline lysis” method, in which bacteria are resuspended in lysis ...
Transfection-grade plasmid isolation kits are specifically designed for scientists who need to extract plasmid DNA (pDNA) with minimal levels of endotoxins from bacterial cultures. During the plasmid purification process, large amounts of endotoxins are released from lysed bacteria, which c...
Transfection-grade plasmid isolation kits are specifically designed for scientists who need to extract plasmid DNA (pDNA) with minimal levels of endotoxins from bacterial cultures. During the plasmid purification process, large amounts of endotoxins are released from lysed bacteria, which can negatively...
Isolation of nucleic acid using colored buffers The present invention describes isolation of plasmid DNA from bacteria. The addition of dyes to the alkaline lysis based purification buffers (P, P, and P) allows for improved visual monitoring of the steps of preparing a bacterial lysat... X Jia...
HeLa Cells seeded in a 6-well plate were transfected with either 2 or 4 µg of pCI-neo®+GFP plasmid isolated from 100 ml of bacteria culture using the ZymoPURE Midiprep Kit, ZymoPURE Maxiprep Kit, or MN Midiprep Kit. GFP expressin was assessed 48 hours later in cell lysates using...
Isolation of the pCTD plasmid from C.trachomatis G0/86 C.trachomatis cells were isolated following known techniques from the urethra of a patient with non-genococcic urethritis. The strain, identified as serotype D by the micro-immunofluorescence technique described by Wang, S.P. and Grayston...
An improved method for rapid isolation of plasmid DNA from wild-type gram-negative bacteria for plasmid restriction profile analysis. An improved method for the isolation of large and small plasmids from wild-type Gram-negative bacteria has been developed. The protocol combines the lysis ... JE ...
aDistribution of parameter values obtained using Bayesian inference to estimate growth kinetic parameters from OD measurements obtained for each strain in isolation. Diamonds representKlebsiellaspp. strains and circlesE. coliclones; filled symbols denote plasmid-bearing strains and empty symbols plasmid-free...
An expedient procedure for large-scale plasmid isolation from Escherichia coli strains without using ultracentrifugation or special setups or reagents is d... A Chakrabarti,S Sitaric,S Ohi - 《Biotechnology & Applied Biochemistry》 被引量: 9发表: 2011年 A Rapid Procedure for Screening and Isolati...