几适度的谷物需要开花结实促进法支持 flowering.然而,很少对 vernalization-memory-related 基因被知道,并且几乎,没有比较分析被执行了.这里, RNA-Seq 被用于 transcriptome 分析在非使加速开花结果,使 \\O 加速开花结果并且 vernalized 以后 Brachypodium distachyon (L.) Beauv.幼苗.总共, 1,665 基因的表示响应开花...
We employed next-generation RNA sequencing (RNA-Seq) technology to determine the influence of obesity on global gene expression in skeletal muscle feed arteries. Transcriptional profiles of the gastrocnemius and soleus muscle feed arteries (GFA and SFA, respectively) and aortic endothelial cell-enriched...
As a general rule, RNA degradation starts with an endonucleolytic processing in the RNA body, leading to the generation of decay intermediates. Those are further digested by exoRNases and, finally, by oligoRNase/nanoRNases1. The main endoRNases that have been demonstrated to initiate RNA d...
Alternative RNA structures such as RNA G-quadruplexes (rG4s) are often important in gene regulation and cellular processes, however, there is no experimental method for transcriptome-wide assessment of rG4 structures. Here, we introduce a novel approach called rG4-seq, which marries rG4-mediated...
genes by RNA-seq in CD patients [154,155]. Unfortunately, there was no susceptibility gene overlap between RNA-seq results and TWAS results neither in the ileum or colon. And the susceptibility genes screened by TWAS was less overlapped with the results of RNA-seq. There are several reasons...
RNA-Seq analysis was performed in all Input samples, and the overall distribution of DEGs could be understood through the volcano map in Fig.4A. In this study, FPKM was used to measure the abundance of gene expression in different samples. As shown in Fig.4B, this study found that among ...
955 were downregulated, which indicated that high temperature stress significantly altered the methylation pattern of m6A, and there were more demethylation sites in the T32 group. Conjoint analysis of the m6A methylation modification and the transcript expression level (the MeRIP-seq and RNA-seq ...
RNA sample quality was assessed by NanoDrop and Agilent 2100 BioAnalyzer. Samples with RNA integrity number (RIN) above 7, OD260/280: 2, and OD260/230 ≥ 2 were used. In addition, RNA degradation and contamination were monitored on 1% agarose gels before RNA-Seq [13]. Library ...
Although cGTEx had efficient performance in detecting eQTLs by RNA-seq alone, some SNPs located in non-transcripted regions would not be detected, which might affect the final detection of eQTLs. Therefore, eQTL mapping needs to be performed in a more refined tissue and a specific breed in ...
RNA-binding proteins Leishmania OOPS TMT labeling LC-MS/MS RNA-seq non-Poly(A) interactome WDR domain INTRODUCTION Unicellular parasites of the genus Leishmania are the causative agents of leishmaniasis, a major vector-borne infectious disease in 98 tropical and subtropical countries (1). Being evo...