PrediXcan , SPrediXcan,MetaXcan是近些年基于GWAS后续分析开发出来的工具。 主要功能是在组织和表达的层面预测影响表型的基因,弥补了GWAS只能在基因组层面解释表型的不足。 下面是这几个工具的工作流程: 今天给大家介绍一下如何使用SPrediXcan和MetaXcan工具进行全转录组分析(Transcriptome-Wide Analysis) 该工具最大的优点...
Understanding of the transcriptome-wide landscape is conducive to discover the mechanism associated with placental morphological and functional changes. Similar with previous transcriptome or proteome studies, we also found that DEGs between abnormal and normal placentas were functionally enriched in complement...
Fig. 3: Transcriptome-wide association study (TWAS) for SPP and enrichment analysis of TWAS significant genes. aManhattan plot of TWAS for SPP. TWASZ-scores (y-axis) are plotted against gene positions (x-axis) on each of the chromosomes. The gray dashed lines indicate theZ-scores at the ...
The bottom panel shows a Manhattan plot of the GWAS data before (gray) and after (blue) conditioning on the green genes. You can see that this locus goes from being genome-wide significant to non-significant after conditioning on the predicted expression of FAM109B. 蓝色和绿色基因均是通过TWA...
[4,11–15,79]. As a step towards understanding genes influential in the expression of specific phenotypes throughout an individual's life cycle stage, we performed a transcriptome-wide analysis of individual specimens of the pre-adult stages, castes and sexes of the bumblebee to improve our ...
Transcriptome-wide association analysis is a powerful approach to studying the genetic architecture of complex traits. A key component of this approach is to build a model to impute gene expression levels from genotypes by using samples with matched genotypes and gene expression data in a given tiss...
Crohn’s disease (CD) is regarded as a lifelong progressive disease affecting all segments of the intestinal tract and multiple organs. Based on genome-wide association studies (GWAS) and gene expression data, transcriptome-wide association studies (TWAS
Transcriptome-wide analysis of small RNA expression in early zebrafish development. RNA. 2012;18(5):915–29. Article CAS PubMed PubMed Central Google Scholar Ruan J, Dean AK, Zhang AW. A general co-expression network-based approach to gene expression analysis: comparison and application. BMC...
Harr B, Turner LM: Genome-wide analysis of alternative splicing evolution among Mus subspecies. Mol Ecol. 2010, 19 (Suppl 1): 228-239. Article CAS PubMed Google Scholar Kelemen O, Convertini P, Zhang Z, Wen Y, Shen M, Falaleeva M, Stamm S: Function of alternative splicing. Gene. ...
Transcription factors (TFs) are vital elements that regulate transcription and the spatio-temporal expression of genes, thereby ensuring the accurate development and functioning of an organism. The identification of TF-encoding genes in a liverwort, Marc