Hence, high-throughput methods that allow transcriptome-wide mapping of m1A will be important for further functional investigations. Here, we describe a technique called "m1A-ID-Seq", which is based on m1A immunoprecipitation and the inherent ability of m1A to stall reverse transcription, to ...
Transcriptome-Wide Mapping of N-Methyladenosine by mA-Seq. A detailed protocol for isolation and sequencing of an enriched population of m(6)A-methylated RNA fragments to create m(6)A methylome maps is outlined. Ou... D Dominissini,S Moshitch-Moshkovitz,N Amariglio,... - 《Methods in ...
Recent transcriptome-wide mapping of UPF1 binding sites reveals evidence for its recruitment to mRNA before translation. Translation. 2013;1(2):e26977.Pal M, Ishigaki Y, Nagy E, Maquat LE. Evidence that phosphorylation of human Upfl protein varies with intracellular location and is mediated by...
Erratum: iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution A correction to the article iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution... Issue - 《Jove》 被引量: 184发表: 2011年 Mapping protein–...
transcriptome-wide marker gene panel mapping and gene clustering analysis to decipher the stress mechanisms in four SRB. Here, the accessible RNA-sequencing data from the public domains were mined to identify the key transcriptional signatures. Crucial transcriptional candidate genes ofDesulfovibriospp. ...
This transcriptome-wide mapping of human eIF4AIII reveals unanticipated aspects of the EJC and broadens its potential impact on post-transcriptional regulation. 展开 关键词: Humans Multiprotein Complexes Eukaryotic Initiation Factor-4A Chromosome Mapping Immunoprecipitation Exons Binding Sites High-Throughput...
Transcriptome-wide mapping of N6-methyladenosine (m6A) at base resolution remains an issue, impeding our understanding of m6A roles at the nucleotide level. Here, we report a metabolic labeling method to detect mRNA m6A transcriptome-wide at base resolution, called ‘m6A-label-seq’. Human and ...
Transcriptome-wide Mapping Reveals Widespread Dynamic-Regulated Pseudouridylation of ncRNA and mRNA Transcriptome-wide pseudouridine mapping reveals extensive, dynamic pseudouridylation of mRNA and noncoding RNA in yeast and human. S Schwartz,D Bernstein,M Mumbach,... - 《Cell》 被引量: 286发表: ...
Transcriptome-wide mapping of N(6)-methyladenosine by m(6)A-seq based on immunocapturing and massively parallel sequencing. N-6-methyladenosine-sequencing (m(6)A-seq) is an immunocapturing approach for the unbiased transcriptome-wide localization of m(6)A in high resolution. To ... D Domini...
(m6A) is the most abundant mRNA modification in mammalian cells, occurring at ~3 modified adenosine residues per transcript. The m6A mapping and biology have been extensively studied recently.2At the physiological level, m6A affects embryonic development, circadian clock, immuno-response, and others....