We performed subcellular compartment fractionation (whole cell, nucleus and cytosol) before RNA isolation in 15 cell lines (Supplementary Table 1) to interrogate deeply the human transcriptome. For the K562 cell line, we also performed additional nuclear subfractionation into chromatin, nucleoplasm and ...
How cells adopt different identities has long fascinated biologists. Signal transduction in response to environmental cues results in the activation of transcription factors that determine the gene-expression program characteristic of each cell type. Tec
Dimethylsulfoniopropionate (DMSP) is a pivotal compound in marine biogeochemical cycles and a key chemical currency in microbial interactions. Marine bacteria transform DMSP via two competing pathways with considerably different biogeochemical implications: demethylation channels sulfur into the microbial food ...
2007. Type-B ARR transcription factors, ARR10 and ARR12, are implicated in cytokinin-mediated regulation of protoxylem differentiation in roots of ... A Yokoyama,T Yamashino,YI Amano,... - 《Plant & Cell Physiology》 被引量: 255发表: 2007年 Genetic study on the cytokinin-associated type-...
A tenfold increase in the ATIII cDNA copy number from the lowest to the highest producing cell line coincided with a 38-fold increase in ATIII mRNA levels, and an 80-fold increase in the amount of intracellular ATIII levels. The data was analyzed using a simple kinetic model. The ...
The CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) gene encodes a MYB-related transcription factor involved in the phytochrome induction of a light-harvesting chlorop... ZY Wang,EM Tobin - 《Cell》 被引量: 2005发表: 1998年 Arabidopsis circadian clock protein, TOC1, is a DNA-binding transcription factor...
In this study, we isolated and characterized GmPTF1, a basic helix-loop-helix (bHLH) transcription factor (TF) gene from soybean (Glycine max) with tolerance to inorganic phosphate (Pi) starvation. Alignment analysis indicated that GmPTF1 and other reported bHLH TFs share significant similarity ...
Each cell cycle event—such as DNA replication, centrosome duplication, and chromosome segregation—is a complex process that requires the coordination of many different proteins acting together to complete the task at hand. In turn, each of these complex events must be coordinately controlled with ...
(see below). Total RNA was extracted from two independently isolated wild type and twotfc6mutant strains at mid-log phase growth in rich media (A6001.0) and processed for RNA-Seq (see Materials and Methods). In total, 196,295,402 strand-specific 100 base reads were generated for the ...
Calcium phosphate stones produced by Madin-Darby canine kidney (MDCK) cells inoculated in nude mice The canine renal distal tubular cell line Madin-Darby canine kidney (MDCK) forms calcium phosphate microliths during a long-term culture in vitro. We ident... T Sakakura,K Fujita,T Yasui,......