Measuring transcription dynamics in living cells using a photobleaching approachdoi:10.1016/j.ymeth.2017.04.007The transcriptional kinetics of RNA polymerase II, the enzyme responsible for mRNA transcription in the nucleoplasm, can be modulated by a variety of factors. It is therefore important to ...
Visualizing transcription factor dynamics in living cells. J. Cell Biol. 217, 1181–1191 (2018). Article CAS PubMed PubMed Central Google Scholar Papantonis, A. & Cook, P. R. Transcription factories: genome organization and gene regulation. Chem. Rev. 113, 8683–8705 (2013). Article CAS...
Regulation of TATA-binding protein dynamics in living yeast cells Although pathways for assembly of RNA polymerase (Pol) II transcription preinitiation complexes (PICs) have been well established in vitro, relatively litt... RO Sprouse,TS Karpova,F Mueller,... - 《Proceedings of the National Acad...
Transcription by RNA polymerase (RNAP) is coupled with translation in bacteria. Here, we observe the dynamics of transcription and subcellular localization of a specific gene locus (encoding a non-membrane protein) in living E. coli cells at subdiffracti
Inferring transient particle transport dynamics in live cells Nat. Methods, 12 (9) (2015), pp. 838-840, 10.1038/nmeth.3483 View in ScopusGoogle Scholar [50] X. Michalet Mean square displacement analysis of single-particle trajectories with localization error: Brownian motion in an isotropic medi...
This study was the first to confirm phase transition-based processes in living organisms. Further studies revealed that PS was involved in other interactions cells, such as those among nucleoli and proteins, and in life-supporting activities, such as DNA repair and neural signaling [57, 58]. ...
Similar to SARS-CoV, SARS-CoV-2 infection starts with virion attachment to the target cells mainly via interactions of the S proteins with host-cell receptor ACE2 [4,59,60,61]. Proteolytic cleavage of the S protein by TMPRSS2 results in structural changes of the S protein that initiates ...
Visualizing transcription factor dynamics in living cells. J. Cell Biol. 217, 1181–1191 (2018). CAS PubMed PubMed Central Google Scholar Neikes, H. K. et al. Quantification of absolute transcription factor binding affinities in the native chromatin context using BANC-seq. Nat. Biotechnol. ...
We imaged transcription in living cells using a locus-specific reporter system, which allowed precise, single-cell kinetic measurements of promoter binding, initiation and elongation. Photobleaching of fluorescent RNA polymerase II revealed several kinet
However, the question of how the stochastic nature of transcription might influence splice site selection and spliceosome assembly has not been addressed, and this coupling has never been directly observed for human genes in living cells. The prevailing view of the spliceosome is that of a high-...