Real -Time RT-PCRSARS-COV-2SerologyThe purpose of this study was to investigate the feasibility of serological total antibody tests combined with RT-PCR for detection of SARS-CoV-2. We conducted a retrospective study in which 375 patients were enrolled during the outbreak of SARS-CoV-2 from ...
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To determine what fraction of these "predicted novel genes" (PNGs) represents expressed human genes, we used a sensitive RT-PCR assay to detect predicted transcripts in 17 tissues and one cell line. Our results indicate that at least 5000–9000 additional human genes which lack similarity to ...
The real-time reverse transcriptase polymerase chain reaction (RT-PCR) test, which detects SARS-CoV-2 viral RNA, was developed to confirm the diagnosis of COVID-19 [1]. However, sensitivity of RT-PCR tests can be affected by viral loads in specimens, timing of sample collection during the...
7123 Background: Measuring DNA in plasma using quantitative RT-PCR is a promising method for surveillance of NSCLC. This strategy has the potential to assess response more accurately or quickly than radiologic tests, or to confirm the success of surgical resection and select patients for adjuvant ...
Validation by real-time quantitative RT-PCR (RT-qPCR) indicated subtle changes in differential gene expression, suggesting a mild response to heat stress. This study used molecular tools to increase our understanding of, and to identify candidate genes involved in, the heat stress response of four...
The number of labs that can perform RT-PCR tests was increased from 2 (before January 24) to 40 (after February 24) and the number of RT-PCR test kits was increased from 200 (before January 24) to 7,000 (after February 4). Two new specific hospi- tals, Huoshenshan with 1,000 ...
To ramp up large-scale, community-based COVID-19 testing, BGI and MGI have teamed up to provide a one-stop, total lab solution as a result of the Emergency Use Authorization (EUA) amendment for BGI’s Real-Time Fluorescent RT-PCR Kit for SARS-CoV-2 Detection issued by the U.S. Food...
Using quantitative reverse-transcription polymerase chain reaction (RT-PCR) on a selected set of 23 differentially expressed genes, and by applying a threshold value and weighting algorithm, these researchers developed a gene expression signature that produced a score that differentiated benign nevi from...
Total RNA was extracted using Magzol and reverse-transcribed (RT) into cDNA (Thermo Scientific) according to the manufacturer’s instructions. Quantitative real time PCR (qPCR) was performed using SYBR Green II Master Mix (Thermo Scientific) in iCycler iQ thermocycler (Bio-Rad). The thermal cycl...