RT-PCR test results were positive during the first menstrual period before admission, turned negative during hospitalization, and then positive again during the second menstrual period after hospital discharge. Conclusions: This case indicates sex hormones may play an important role in SARS-CoV-2 ...
Positive RT-PCR Test Results in Patients Recovered From COVID-19 Lan Lan, MD1;Dan Xu, MD1;Guangming Ye, MD2;et alChen Xia, MS3;Shaokang Wang, MS3;Yirong Li, MD, PhD2;Haibo Xu, MD, PhD1 Author AffiliationsArticle Information
摘 要根据罗湖病毒(Tilapia lake virus,TiLV)编码RNA聚合酶片段1和病毒性神经坏死病毒(Nervous necrosis virus,NNV)编码衣壳蛋白的基因片段,设计2组特异性引物,建立同时检测TiLV和NNV的双重RT-PCR(duplex reverse transcription polymerase chain reaction,dRT-PCR)...
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However, understanding whether the RT-PCR test results are interpreted as quantitative, qualitative, or semi-quantitative is important [14]. Unfortunately, several papers on COVID-19 use the naive Ct values from qualitative RT-PCR as a quantitation unit or use the ΔCt values with incorrect ...
目的:探讨实时荧光RT-PCR方法检测新型冠状病毒(SARS-CoV-2)核酸在新型冠状病毒感染者确诊中的应用。 方法:从中国疾病预防控制信息系统选取截至2020年3月5日录入的河南省COVID-19的实验室检测数据和病例基本信息。所有信息都由当地医院和疾控...
一、RT-PCR操作步骤 1. RNA抽提 方法:TRlzol法。 主要成分:苯酚(具有裂解细胞和变性蛋白的作用)。 RNA抽提前准备:试剂耗材准备(注意RNase free)、样品的准备。 步骤:样品+1ml TRlzol室温裂解10min+200μL氯仿振荡30s后室温静置2-3 min;4℃低温离心(12 000g×20min);400μL上层水相+600μL异丙醇室温沉淀10...
关键词: 新型冠状病毒;核酸提取;逆转录 PCR;内标 中图分类号:R563.1+4 文献标识码:A 文章编号:1009-9727(2021)02-186-05 DOI:10.13604/j.cnki.46-1064/r.2021.02.18 Results comparing on the internal control of RT-PCR on SARS-CoV-2 by two different nucleic acid extraction methods of releasant ...
Results Probables had similar seropositivity and levels of IgG and IgM antibodies as propensity-score matched RT-PCR confirmed COVID-19 patients (60.0% vs 80.0% for IgG, p-value = 0.13; 50.0% vs 72.5% for IgM, p-value = 0.10), but multi-fold higher seropositivity rates than ...
* High purity. >98% homogeneous of the Taq DNA polymerase by SDS gel electrophoresis. No contamination detected in standard PCR test reactions. * Reproducible results. * Reverse transcription and PCR reactions are completed in one tube, no additional tube opening/pipetting operations are required, ...