Technically Speaking: Promega RT-PCR Systems ExplainedHanan Abramovici
Real-time RT-PCR normalisation; strategies and considerations. Genes Immun. 6, 279–284 (2005). 6. Gutierrez, L. et al. The lack of a systematic validation of reference genes: a serious pitfall undervalued in reverse transcription- polymerase chain reaction (RT-PCR) analysis in plants. Plant...
In general, relative expression changes by real-time RT-PCR were greater than those by microarray analysis, especially for genes that were markedly overexpressed. This may be explained by the better sensitivity and reliability of real-time RT-PCR compared to microarray, whose drawback is the ...
PCR Since the intron-9c.1210-34TG11-12T5 alleles are known to be associated with high rates of exon-10 skipping (and hence with low wild-type CFTR expression levels), we explored the possibility that the phenotype in patients 2 and 5 could indeed be explained by the presence of the T5...
which were a slightly higher than that reported by Peck, who also measured CK19 mRNA, but lower than that measured by CEA RT-PCR preoperatively in Kuresu’s 103 patients with resectable NSCLC27. These discrepant results could be in part explained by differences in RNA extraction, PCR methodol...
Detecting SARS-CoV-2 at point of care: preliminary data comparing loop-mediated isothermal amplification (LAMP) to polymerase chain reaction (PCR) 10.1186/s12879-020-05484-8 Google Scholar [29] X. Hu, Q. Deng, J. Li,et al. Development and clinical application of a rapid and sensitive loop...
To test our platform’s capability to perform multiplex single-cell RT-PCR on living cells encapsulated in microdroplets, three murine cell lines with various gene expression profiles, namely B62c, D4T, and EL4, were assayed. No issues were observed when encapsulating the cell lines despite the...
All assays were run as separate reactions as a 4-well test including the internal control in 384-well plates in a Roche LightCycler 480 (Roche, Indianapolis, IN). The one-step RT-PCR amplification was performed with same PCR conditions described previously30, and mentioned in Table 1. The ...
The ID™ SARS-CoV-2/UK/SA Variant Triplex (ID triplex) assay is a multiplex RT-PCR assay that detects the B.1.1.7, B.1.351, and P1 variants by targeting the 69–70 deletion and the N501Y mutation [23]. Both targets are detected in the B.1.1.7 variant, while only the N501Y ...
For the second case, inhibition during RT-PCR reaction or difference in the melting temperature used by the two laboratories could explain the result. For this last sample, the melting curve was also significantly different from those usually expected for PoSaV. This could be explained by the ...