To further understand the adaptability of our assay in diagnostic and hospital laboratories, we validated the method in three additional commonly used RT-PCR instruments with melt capability. The performance of these new test instruments was compared to the LC480 as the gold standard. A total of ...
Touchdown-quantitative real-time PCR (TqPCR) The TqPCR was carried out as described [13], [14], [19]. Briefly, total RNA or rRNA-depleted samples were subjected to RT reactions using hexamer and M−MuLV Reverse Transcriptase. The RT/cDNA products were further diluted and used as PCR te...
Real-time RT-PCR normalisation; strategies and considerations. Genes Immun. 6, 279–284 (2005). 6. Gutierrez, L. et al. The lack of a systematic validation of reference genes: a serious pitfall undervalued in reverse transcription- polymerase chain reaction (RT-PCR) analysis in plants. Plant...
Using a genomics technology of high throughput quantitative PCR arrays, Vass et al. [9] tested 625 cytotoxic compounds for neighborhood behavior in human hepatic cells. The nature of the compounds ranged from pesticides to hormone mimickers to potential anti-cancer drugs, with a common ...
To date, real-time reverse-transcription PCR has been one of the most promising detection methods due to its sensitivity, specificity and ability to deliver quantitative data in food samples, but it does not provide HAV subtyping information. Results Six subtype-specific RT-qPCR assays were ...
Technically Speaking: Promega RT-PCR Systems ExplainedHanan Abramovici
RT-qPCR data for 18080 protein-coding genes were generated in the context of the Sequencing Quality Control study (SEQC) (17) using PrimePCR assays (BioRad) (Supplemental Table 1). In order to define the ensemble of transcripts amplified by every individual qPCR assay, assays were re-mapped...
Acanthamoebae are potentially pathogenic organisms, with a highly unique, yet still insufficiently investigated metabolism. Many open questions can be addressed by gene expression studies, however, for Acanthamoeba reliable standards have not yet been es
To evaluate the efficacy of HCV RNA detection in clinical samples, the results obtained from the developed RT-LAMP-coupled CRISPR–Cas12 assay were compared with a commercial real-time RT-PCR method (COBAS AmpliPrep/COBAS TaqMan HCV Test; Roche Molecular Systems, Pleasanton, CA, USA). Plasma ...
RNA extracts from each in vitro plant were used for both targeted molecular test (RT-PCR) or high-throughput sequencing. In addition, two alien external controls were used. They corresponded to plant samples infected at high concentration by a plant virus, called the alien plant virus, which ...