Whole-transcriptome analysis with total RNA sequencing (RNA-Seq) detects coding plus multiple forms of noncoding RNA. Total RNA-Seq can accurately measure gene and transcript abundance, and identify known and novel features of the transcriptome. ...
total RNA‐seqcomplete transcriptome analysisillumine sequencing‐by‐synthesis sequencingmethodsdata collectionapplicationsWe have developed a new protocol that enables sequencing of the complete transcriptome from small amounts of total RNA regardless of sample origin or quality. The method uses a ...
Here, we performed RNA sequencing (RNA-seq) on nine motor cortex and six cerebellar autopsy samples from RTT patients and controls. This approach identified 1887 significantly affected genes in the motor cortex and 2110 genes in the cerebellum, with a global trend toward increased expression. ...
RNA (RNA instability; cost and complexity of reverse transcription reaction; etc.), but these are not unlike standard RNA-sequencing for transcriptomics. The appropriateness of using RNA for the characterization of microbial communities has been debated as it is assumed that RNA content is linked ...
RNA profiling has emerged as a powerful tool to investigate the biomarker potential of human biofluids. However, despite enormous interest in extracellular nucleic acids, RNA sequencing methods to quantify the total RNA content outside cells are rare. Here, we evaluate the performance of the SMARTer...
Nanodigmbio Technology provides a total solution for RNA-Cap sequencing on both MGI platforms and Illumina® platforms.
Point mutation analysis in a mammalian gene: rapid preparation of total RNA, PCR amplification of cDNA, and Taq sequencing by a novel method. We have developed a very rapid procedure for DNA sequence analysis of induced mutations in a typically large mammalian gene. We are able to determine ...
High-throughput complementary DNA sequencing (RNA-Seq) is a powerful tool for whole-transcriptome analysis, supplying information about a transcript's expression level and structure. However, it is difficult to determine the polarity of transcripts, and therefore identify which strand is transcribed. He...
identify spatially defined expression of noncoding transcripts in skeletal muscle regeneration and highlight host transcriptional responses associated with local viral RNA abundance. STRS requires adding only one step to the widely used Visium spatial total RNA-sequencing protocol from 10x Genomics, and ...
allows for both coding and non-coding RNA sequencing making it distinct from other transcriptome sequencing methods. Instead of undergoing the polyA+ selection, this technique subjects the entire RNA to rRNA depletion. This is because the rRNA makes the biggest percentage of the whole RNA. ...