今天小编和大家分享一篇22年11月发表在Frontiers in Immunology(IF:8.786)杂志的文章《Identification and validation of a signature based on macrophage cell marker genes to predict recurrent miscarriage by integrated analysis of single-...
RNA芯片和测序技术的比较 前面我们介绍了表达量研究领域的,基因芯片和RNA-seq测序技术,并且把详细的学习资料和视频教程免费共享在了B站。 有学员提出来了一个问题,就是可以比较 同样实验设计的表达量探索研究,一… 曾健明发表于生信技能树 Nat Comm | 深度清洗单细胞RNA-Seq数据的通用深度神经网络 图灵基因发表于前沿...
Single-cell sequencing provides rich information; however, its clinical use is limited due to high costs and complex data output. Here, we present a protocol for extracting single-cell-related information from bulk RNA sequencing (RNA-seq) data using the pathway-level information extractor (PLIER)...
As library costs and sequencing costs drop, standard RNA isolation becomes a considerable factor for the cost efficiency of RNA-seq methods. RNA isolation using magnetic beads is an attractive alternative [50] and we have used it successfully in combination with our protocol before [11]. To inve...
今天小编和大家分享一篇22年11月发表在Frontiers in Immunology(IF:8.786)杂志的文章《Identification and validation of a signature based on macrophage cell marker genes to predict recurrent miscarriage by integrated analysis of single-cell and bulk RNA-sequencing》。作者通过单细胞和bulk RNA测序的联合分析,...
Bulk RNA-sequencing pipeline流程(含代码) 对于Bulk RNA-seq测序(用于比较转录组学,如不同物种的同种组织样本,也就是我们常说的常规转录组测序,注意和单细胞测序区分),我们常用的分析流程有很多,之前的文章也有介绍。偶然在github上发现一个总结的很完整的分析流程和大家分享。
RNA-sequencing (RNA-seq) has become an increasingly cost-effective technique for molecular profiling and immune characterization of tumors. In the past decade, many computational tools have been developed to characterize tumor immunity from gene expression data. However, the analysis of large-scale RNA...
Prognosis, immunological features and potential mechanisms of HKR1 in prostate cancer via single-cell and bulk RNA-sequencing doi:10.1186/s12885-025-14230-9HKR1Prostate cancerImmunotherapyPrognosisScRNA-sequencingGiven the limitations of conventional therapies in prostate cancer (PCa) management, identifying...
Bulk RNA-sequencing pipeline流程(含代码) 2020/11/12 添加评论 对于Bulk RNA-seq测序(用于比较转录组学,如不同物种的同…© 2025 Omics – Hunter 浙公网安备 33010802011761号 陕ICP备19016588号-1 邮箱:chenhao__@__evvail.com(发件请删除下划线) 站点地图 | RSS订阅 | 关于我 | 文章总览 | 友链:...
Quality control of single-cell RNA sequencing data was executed with the following criteria: > 200 genes/cell, < 3000 genes/cell, > 3 cells/gene, and < 20% mitochondrion genes. The batch effect of 9 samples was eliminated using IntegrateData of Seurat packages [32]. The...