Influence of silibinin (0.3, 1 μg/mL) on expressions of total cytokine genes in two human hepatocellular carcinoma (HCC) cell lines with different characteristics (cell lineA, HCC24/KMUH, without susceptible to amphotericin B-induced oxidative stress; cell lineB, HCC38/KMUH, susceptible to ...
Above the exons, chromosomal positions based on the University of California Santa Cruz genes and the hg18 version of the genome are shown. The source of the sample RNA (K562, liver or brain) and the type of RNA preparation (RiboMinus or polyA selected) are shown next to the Y-axis. ...
whole cells (total RNA) were prepared from three human cell lines and sequenced using massive parallel sequencing./p pResults/p pFor all three cell lines, of about 15000 detected genes approximately 400 to 1400 genes were detected in different amounts in the cytoplasmic and total RNA fractions...
Mini-preparation of total RNA for RT-PCR from cultured human cells Isolation of undegraded total RNA is very important for examining the transcriptional levels of genes in different in vitro systems. Guanidinium isothiocya... B Ogretmen,AR Safa - 《Biotechniques》 被引量: 46发表: 1995年 ...
XVI. The Complete Sequences of 150 New cDNA Clones from Brain Which Code for Large Proteins in vitro We have carried out a human cDNA sequencing project to accumulate information regarding the coding sequences of unidentified human genes. As an extension of the preceding reports, we herein ...
IL13, IL4, IL4RA, FCER1B and ADRB2 are susceptible genes of asthma and atopy. Our previous study has found gene–gene interactions on asthma between these genes in Chinese Han children. Whether the interactions begin in fetal stage, and whether these gen
Consensus human leukocyte interferon Described are rapid and highly efficient procedures for the total synthesis of linear, double stranded DNA sequences in excess of about 200 base pairs in length, which sequences may comprise entire structural genes. Novel sequences are p... Y Stabinsky - US ...
With current limitations in our knowledge of marker genes across cancers, total mRNA expression per tumor cell may represent a robust and measurable pan-cancer feature that warrants a systematic evaluation in patient cohorts. Measuring such a feature in human tissues at-scale poses several analytical...
Intestinal epithelial cells could quickly repair injured mucosa through modifying the patterns of expressed genes. The activation of transcription factors is essential to control a range of specific genes expression that regulate cell proliferation, migration, junctions and epithelial barrier. c-Myc as a...
Ira Herskowitz (Univ. California, San Francisco), in the first talk of the meeting, laid out the ways in which directive use ofthe yeast system can benefit mammalian studies: i) by using the structural similarity of yeast and human genes, ii) by mak- ing functional assays of mammalian ...