For the optimization of the codon of the chimeric peptide inE. coli(strain K12) for maximum exhibition of protein, the Java Codon Adaptation Tool (JCat) was employed. The codon sequence optimized by the tool had a length of 1500 nucleotides. CAI also known as the Codon Adaptation Index was ...
All procedures for RNA-seq were performed according to the instructions from the manufacturer, Illumina. The RNA library was constructed using the the Agilent's SureSelect Strand Specific RNA Library Preparation Kit, accompanied by AMPure XP Beads size selection. Paired-end sequences with a read ...
The formation of this locus can occur by various mechanisms such as, for example, the slippage of one DNA strand during DNA replication or the reinsertion of replicate copies from extrachromosomal circular DNA intermediaries. In a second step, this repetitive family can be disseminated throughout ...