吉满生物H_TNFR2 Reporter V2 Cell Line是一株敲除了H_TNFR1的Luciferase报告基因细胞系。当mTNF-α与TNFR2结合时,导致下游通路激活;加入拮抗剂后,阻断下游信号通路,进而导致荧光素酶(Luciferase)的表达变化。Luciferase读值即代表信号通路的激活效果,因此可用于TNFR2拮抗剂的体外效果评价。
TNFR2 signaling activity was measured in a HEK-Blue NFkB reporter cell line and also by phosphorylated RelA (pRelA) induction in primary human Tregs. In functional assays, human PBMCs were incubated with the anti-TNFR2 agonistic antibody for 5 days for assessment of Treg proliferation and ...
RNAi-transfected cells were used for experi- ments, including the SEAP reporter assay for functional NF-kB activation and quantification of cell proliferation as described above. For NF-kB reporter assays, the reporter plasmid was co-transfected with the RNAi. Each experiment was performed three ...
Introduction Polymorphonuclear neutrophils(PMNs) represent the first line of defense against invadingpathogensand are involved in inflammatory processes. PMNs kill bacteria by releasing anti-bacterial proteins (degranulation), production ofreactive oxygen species(ROS) (oxidative burst), and byphagocytosis. Ano...
B: FACS analysis of TNFR1 and TNFR2 cell surface protein expression of parental HEK293 cells or HEK293 cells stably expressing TNFR2 or TNFR2 plus NF-κB-luciferase reporter construct. Unfortunately, our attempts to transiently transfect KYM-1 cells were unsuccessful. We discovered that KYM-1 ...
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