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An iNKT reporter cell line (JE6-1) was engineered by introducing the human iNKT-TCR into a human leukemic T cell line carrying an NF-κB-driven fluorescent transcriptional reporter construct. Antigen presenting BWcells expressing human CD1d and CD80 were generated. Reporter induction in JE6-1...
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The well-established murine hybridoma cell line DN32.D337 stained positive for both, the iNKT-TCR and CD28 (Fig. 1B –right panel), in contrast to the control cell line N37-1A1238, which stained positive only for CD28. Figure 1 Generation of an iNKT-TCR-transgenic reporter T cell line....
Generation of Red-COL1A1 reporter cell line The parental BJ-hTERT fibroblasts were electroporated using Neon Transfection System (Thermofisher) with equal amounts (3 µg) of plasmids encoding for both TALENs and the donor plasmid. Electroporated cells were expanded, and recombinant cells express...
This reporter plasmid was stably trans‐fected into NH3T3 cells for generation of reporter cell line. When the reporter cell line was infected with E2F‐1 together with excess amounts of empty vector, the cells that received the positive modulator were readily identifiable by green fluorescence ...
2.3. Generation of A549 control cell lines overexpressing CMV-EGFP or CAG-EGFP-ABCG2 A control A549 cell line, expressing a CAG-EGFP-ABCG2wt construct, was generated using the Sleeping Beauty transposon gene expression system. Plasmids encoding the transposase and the EGFP-ABCG2wt protein were...
The cell line has been screened using the MycoAlert™ Mycoplasma Detection kit (Lonza, #LT07-218) to confirm the absence of Mycoplasma species. Background GM-CSF is a cytokine that can stimulate the generation of myeloid cells such as granulocytes, neutrophils, and monocytes from its bone mar...
cell line generation(4)(5)(6). By selecting the right pGL4 Vector for your system and using any of ourLuciferase Assay Systems, you can create an ultrasensitive assay that enables the accurate probing of the regulation and activity of a protein or a pathway inside the cell or in a cell...
Cell line repository/bank N/A Ethical approval The choice of appropriate human donors, the procedures for skin biopsy, isolation of dermal fibroblasts, generation of iPSCs and their use in further scientific investigations were performed under the positive vote of the Ethics Committee of the Medical...