qPCR Instruments 规格 5 X 96 Tests 组份 - 分析稀释缓冲液 - 抗体结合物 A - 抗体结合物 B - 抗体结合稀释缓冲液 - 标准品 - 预混料 - 连接酶 储存温度 -20℃ 运输条件干冰 Protein nameTNF alpha Protein aliases tumor necrosis factor 物种(Tested) ...
<0.02 ng/μg of protein tested by qPCR. 无菌(Sterility) The sterility testing was performed by membrane filtration method described in CP<1101>, USP<71> and Eur. Ph. 2.6.1. 支原体(Mycoplasma) Negative. 纯度(Purity) >95% as determined by SDS-PAGE. >95% as determined by SEC-HPLC. ...
qPCR Instruments 产品规格 96 Tests 产品成分 Assay Dilution BufferAntibody-Conjugate AAntibody-Conjugate BAntibody-Conjugate Dilution BufferStandardMaster MixLigase 运输条件 Dry ice 储存 -20°C 蛋白名称 TNF alpha 均相(无需洗涤) Human 检测试剂盒格式 Proximity-based Immunoassay 标签或...
Expression of IFN-target genes was measured by real-time quantitative reverse transcription-polymerase chain reaction (qPCR) and enzyme-linked immunosorbent assay (ELISA). Total STAT1 protein and IFN-mediated STAT1 activation were evaluated by Western blotting. Total histone levels, histone acetylation, ...
In HL-60 cells, inhibition of TNF-alpha reduced the expression of p65; in addition, inhibition of p65 reduced the expression of TNF-alpha as assessed by RT-qPCR and western blot analysis. p65 and TNF-alpha were highly expressed in AML patients, and these 2 molecules were strongly correlat...
使用中注意事项开盖使用前,请先离心。本产品为冻干粉产品,推荐溶解Human TNF-alpha蛋白质的溶液为无菌的ddH20,且浓度不低于100µg/ml,客户可根据后续的实验需求,进行进一步的稀释。 保存建议本产品为冻干粉形式,厂家建议:将冻干粉的【TNF】重组人肿瘤坏死因子-alpha产品保存于-18℃以下的干燥环境中。本产品溶解之...
【PCR/RT-PCR/qPCR】PCR试剂 PCR对照 特异性PCR试剂盒 PCR克隆试剂盒 RNA 载体及构建 PCR克隆载体 M13克隆载体 细菌克隆载体 文库及构建 cDNA文库 基因组文库 噬菌体展示文库【酶】限制性内切酶 蛋白酶 核酸酶 激酶 聚合酶 连接酶【cDNA及合成纯化】cDNA全长基因 RNA cDNA合成 cDNA相关试剂【核酸/蛋白合成】...
【PCR/RT-PCR/qPCR】PCR试剂|PCR对照|特异性PCR试剂盒|PCR克隆试剂盒|RNA 载体及构建|PCR克隆载体|M13克隆载体|克隆载体|文库及构建|cDNA文库 基因组文库 噬菌体展示文库【蛋白检测】Western印迹|报告基因检测|蛋白检测试剂盒 【克隆与表达】克隆基因|克隆试剂盒|克隆筛选【核酸/蛋白合成】Oligo纯化|核酸合成柱|核酸...
(a) The presence of the major intestinal cell types where analyzed by qPCR for specific intestinal markers: intestinal stem cells (Lgr5), enterocytes (Villin); Paneth cells (Lysozyme), Goblet cells (Mucin 2) and enteroendocrine cells (Chromogranin A). The mRNA levels of each marker were ...
In this study, we investigated the expression of isoforms of TNFR1 and TNFR2 receptors before and after exposure to TNFα in two cell lines that had previously demonstrated diverse effects on cell proliferation under TNFα incubation using RT-qPCR. We found that after incubation with TNFα: (...