However, the weakness still exists in Tn5 transposase that hinders its further application. This article primarily reviews the architecture, catalytic mechanism, and functional modification of Tn5 transposase, as well as its application in advanced transgenic editing and library construction of sequencing....
Peopledidn’thaveenoughknowledgeabout itstranspositionmechanism ,thehotspotofTn5insertionandotherconcernedissues.W ithsuccessfully constructingahyperactivetransposaseinvitrowhichcontainsthepointmutationsE54K and1.372P,and furthering theresearch on the three— dimensionalstructuresofthecatalytic core domainsofTnp,the ...
以转 座酶为 载体研究染色质可及性的技术 ( assay for transposase accessible chromatin with high-throughput sequencing,ATAC-seq) 在这一领域有着极为重要的 地位. 该技术利用 Tn5 转座酶优先结合 DNA 中未 受核小体保护的区域的特点,将测序接头引入染色 质的松弛区域进行测序,以测定全基因组的染色质 可及...
Tn5 transposase (Tnp), a 53.3-kDa protein, enables the movement of transposon Tn5 by a conservative mechanism. Within the context of a protein and DNA synaptic complex, a single Tnp molecule catalyzes four sequential DNA breaking and joining reactions at the end of a single transposon. The ...
以转座酶为载体研究染色质可及性的技术(assay for transposase accessible chromatin with high-throughput sequencing,ATAC-seq)在这一领域有着极为重要的地位。该技术利用Tn5转座酶优先结合DNA中未受核小体保护的区域的特点,将测序接头引入染色质的松弛区域进行测序,以测定全基因组的染色质可及性[36]。相比以往染色...
International Journal of Molecular Sciences Review Tn5 Transposase Applied in Genomics Research Niannian Li 1,†, Kairang Jin 1,†, Yanmin Bai 2,†, Haifeng Fu 1, Lin Liu 1,* and Bin Liu 3,4,* 1 College of Life Sciences, Nankai University, Tianjin 300071, China; liniannian123liu...
The inhibitor protein lacks the N-terminal domain of transposase. Thetop half of the figure suggests a mechanism for inhibition. The interaction between the C-terminal domains of the inhibitor protein (top left) is preserved in the inhibited complex containing one molecule of inhibitor protein and...
the specific 19-bp Tn5 end sequences, and target DNA; transposase may not be able to self-dissociate from product DNAs; Tn5 transposes by a conservative "cut and paste" mechanism; and Tn5 release from the donor backbone involves precise cleavage of both 3' and 5' strands at the ends of...
Prokaryotic transposon (Tn) serves as a model system for studying the molecular mechanism of DNA transposition. Elucidation of the X-ray co-crystal structu... M Steiniger-White,I Rayment,WS Reznikoff - 《Current Opinion in Structural Biology》 被引量: 155发表: 2004年 Structure/function insights...
(Dam) of the transposase promoter, which diminishes transcription during most of the cell cycle of the transposase gene, but not the embedded inhibitor gene, thereby helping link transposition to DNA replication; (iii) preferential action by transposase on Tn5/IS50DNA ends very close to the gene...