图1 Tn5 转座机制的示意图（来源：Tn5 Transposase Applied in Genomics Research）关于转座酶建库 由于Tn5具备独特的“切割和粘贴”功能，它在基因组学研究中得到了广泛应用。研究发现，体外转座反应仅需要OE序列和Tn5转座酶。Tn5转座酶能够随机将接头或条形码插入DNA中，生成的DNA分子可以直接用于PCR
[2]Li N, Jin K, Bai Y, et al. Tn5 Transposase applied in Genomics Research [J]. International journal of molecular sciences, 2020, 21(21): 8329. [3]Steiniger-White M, Rayment I, Reznikoff W S. Structure/function insights into Tn5 transposition [J]. Current Opinion in Structural Biolo...
[1] Rice P A , Baker T A . Rice PA, Baker TA. Comparative architecture of transposase and integrase complexes. Nat Struct Biol 8: 302-307[J]. Nature Structural Biology, 2001, 8(5):302-307.[2] Li N , Jin K , Bai Y , et al. Tn5 Transposase Applied in Genomics Research[J...
[1] Rice P A, Baker T A. Comparative architecture of transposase and integrase complexes. Nature Structural Biology 8: 302-307[J]. Nature Structural Biology, 2001, 8(5):302-307.[2] Li N, Jin K, Bai Y, et al. Tn5 Transposase Applied in Genomics Research[J]. International...
[1] Rice P A , Baker T A . Rice PA, Baker TA. Comparative architecture of transposase and integrase complexes. Nat Struct Biol 8: 302-307[J]. Nature Structural Biology, 2001, 8(5):302-307. [2] Li N , Jin K , Bai Y , et al. Tn5 Transposase Applied in Genomics Research[J]....
[2] Li N, Jin K, Bai Y, et al. Tn5 Transposase applied in Genomics Research [J]. International journal of molecular sciences, 2020, 21(21): [3] Steiniger-White M, Rayment I, Reznikoff W S. Structure/function insights into Tn5 transposition [J]. Current Opinion in Structural Biology,...
[2]Li N, Jin K, Bai Y, et al. Tn5 Transposase applied in Genomics Research [J]. International journal of molecular sciences, 2020, 21(21): 8329. [3]Steiniger-White M, Rayment I, Reznikoff W S. Structure/function insights into Tn5 transposition [J]. Current Opinion in Structural Biolo...
[2] Li N, Jin K, Bai Y, et al. Tn5 Transposase applied in Genomics Research [J]. International journal of molecular sciences, 2020, 21(21): [3] Steiniger-White M, Rayment I, Reznikoff W S. Structure/function insights into Tn5 transposition [J]. Current Opinion in Structural Biology,...
International Journal of Molecular Sciences Review Tn5 Transposase Applied in Genomics Research Niannian Li 1,†, Kairang Jin 1,†, Yanmin Bai 2,†, Haifeng Fu 1, Lin Liu 1,* and Bin Liu 3,4,* 1 College of Life Sciences, Nankai University, Tianjin 300071, China; liniannian123liu...
[2]Li N, Jin K, Bai Y, et al. Tn5 Transposase applied in Genomics Research [J]. International journal of molecular sciences, 2020, 21(21): 8329. [3]Steiniger-White M, Rayment I, Reznikoff W S. Structure/function insights into Tn5 transposition [J]. Current Opinion in Structural Biolo...