mini-Tn5taggingRecently the gfp (green fluorescent protein) gene from the jellyfish Aequoria victoria has been widely used as a reporter gene. In this study mini-transposons, named as mini-Tn5gfp, were constructed by subcloning the gfp gene into a transposon Tn5. To improve the expression ...
通过位于质粒cis上的tnp*基因编码的tansposase(转座酶)的作用,mini-Tn5可被从运载体质粒上转置到染色体上。pUT mini-Tn5载体,可通过在mini-Tn5区域内进行克隆,从而在细菌染色体内插入任何一段DNA片段。在插入片段后,tnp*基因会缺失。因此,mini- transposon就会稳定的整合到染色体中,并获得稳定遗传。不同的pUT mini...
鸡白痢沙门氏菌Mini-Tn5转座子插入突变
2) Mini-Tn5 transposon Mini-Tn5转座 1. Objective: To express sorbose dehydrogenase(SDH) in fast growing Ketogulonigenium-like strain Y25K2 with Mini-Tn5 transposon. 目的:利用Mini-Tn5转座系统在葡糖杆菌中表达山梨糖脱氢酶(SDH)。3) Tn5 transposon Tn5转座子 1. The green fluorescence protein...
Growth-deficient mutants of Aeromonas hydrophila PPD 134/91 were induced by transposon mini-Tn 5 mutagenesis, with Escherichia coli SM 10lambdapir (pUT:: mini-Tn 5Km) as the Tn 5 donor. Growth-deficient mutants were distinguished by their abilities to grow on TSA plates supplemented with ...
Q. (1999). A bifunctional transposon mini-Tn5gfp-km which can be used to select for promoter fusions and report gene expression levels in Agrobacterium tumefaciens. FEMS Microbiol Lett 179, 37-42.TANG X , LU B F , PAN S Q. Abifunctional transposon mini2 Tn5 gfp2km which can be used ...
Growth-deficient mutants of Aeromonas hydrophila PPD 134/91 were induced by transposon mini-Tn5 mutagenesis, with Escherichia coli SM10位pir (pUTmini-Tn5Km) as the Tn5 donor. Growth-deficient mutants were distinguished by their abilities to grow on TSA plates supplemented with tetracycline and ...
1999. Bi- functional gfp- and gusA-containing mini-Tn5 transposon derivatives for combined gene expression and bacterial localization studies. J. Microbiol. Methods 35:85-92.Xi C , Lambrecht M , Vanderleyden J & Michiels J ( 1999 ) Bi-functional gfp -and gusA -containing mini-Tn5 transposon...
transposon mutagenesisWe previously reported that the viability of Salmonella Oranienburg strains under NaCl stress was variable and depended on the strain's origin; food strains were resistant and patient strains sensitive to NaCl. Therefore, we mutagenized a food strain with a mini-Tn5Km2 transposon...
A magnetosome deleted mutant NM4 of Magnetospirillum gryphiswaldense MSR-1 was generated by mini-Tn5 transposon mutagenesis, and a 5045-bp fragment flanking mini-Tn5 in NM4 was cloned by Anchored PCR. Sequencing analysis showed that this fragment involved six putative open reading frames (ORFs);...