默认下,参考样本的选择是通过比较每个样本的CPM (counts per million)的上四分位数与所有样本CPM的平均上四分位数之间的差值,找出差值最小的样本作为参考样本。 # library size of each sampleslib.size<-colSums(counts1)# library size normalized read count for each gene in each samplecounts1.cpm<-t(t(...
name# --cpm-output CPM_OUTPUT# CPM output file name# --quantile-output QUANTILE_OUTPUT# Quantile-normalized expression output file name 获取有关特定方法的信息,例如CPM: rnanorm cpm --help 使用CPM进行标准化: rnanorm cpm exp.csv --out exp_cpm.csv 文件exp.csv需要是逗号分隔的文件,其中基因在列...
TPM1.0.genes.counts.matrix.TMM_normalizedData from: Differentially expressed genes match bill morphology and plumage despite largely homogenous genomes in a Holarctic songbird.Mason, Nicholas ATaylor, Scott AMason, N. A., and S. A. Taylor (2015). Differentially expressed genes match bill ...