name# --cpm-output CPM_OUTPUT# CPM output file name# --quantile-output QUANTILE_OUTPUT# Quantile-normalized expression output file name 获取有关特定方法的信息,例如CPM: rnanorm cpm --help 使用CPM进行标准化: rnanorm cpm exp.csv --out exp_cpm.csv 文件exp.csv需要是逗号分隔的文件,其中基因在列...
代码语言:javascript 运行 AI代码解释 # Normalized pseudo counts are obtained with the function cpm and stored in a data frame: pseudo_TMM <- log2(cpm(dge2) + 1) df_TMM <- melt(pseudo_TMM, id = rownames(raw_counts_wn)) names(df_TMM)[1:2] <- c ("id", "sample") df_TMM$meth...
默认下,参考样本的选择是通过比较每个样本的CPM (counts per million)的上四分位数与所有样本CPM的平均上四分位数之间的差值,找出差值最小的样本作为参考样本。 # library size of each sampleslib.size<-colSums(counts1)# library size normalized read count for each gene in each samplecounts1.cpm<-t(t(...
CPM (Counts per million) is a basic gene expression unit that normalizes only for sequencing depth (depth-normalized counts). CPM is also known as RPM (Reads per million).The CPM is biased in some applications where the gene length influences gene expression, such as RNA-seq....