For immunocytochemistry, fixed tissue must be cut in thin sections to be viewed in the light or fluorescence microscope. There are two common ways of sectioning tissue – the cryostat for fixed frozen tissue and the rotary microtome for paraffin-embedded tissue. In animal research, select the ...
paraffin-embedded (FFPE) autopsy tissue sections due to delayed fixation. Fixation is a foundational step in the study of pathology and prevents the degradation of tissue and tissue components, ensuring that their
The tissue sectioning, micro-compartmentalization, and micro-specimen procurement All animal experiments were performed according to the protocols approved by the Roswell Park Institutional Animal Care and Use Committee (IACUC). An eight-week-old male healthy Swiss Webster (CFW) mouse was anesthetized ...
By tissue sectioning, we found that the overall morphology of the gut appeared intact and did not exhibit extensive ulcer or cell loss (Figures 3G and 3H), different from that observed in mammalian experiments (see discussion). Instead, we noticed that the basal layer underneath the enterocytes...
To provide an instrument for fabricating a prepared slide of a tissue section and a sectioning method capable of easily changing a direction of a cut face relative to a cut blade while observing the cut face of an embedding block and capable of increasing an efficiency of a rough cutting oper...
That ends the journey from tissue to wax block, which is, I guess, the start of another journey of sectioning, making slides, and immunohistochemistry! Finally, below is a table that highlights the typical main stages of tissue processing for histology. ...
The tissues were fixed by formalin no longer than 48 hours, and then processed to be ready for sectioning. Two serial tissuesectionswith 5 µm thickness are mounted on a SuperFrost Plus glass slide. TissueArray.com histology paraffin tissue section is perfect fo fast detection of genes and ...
We have developed a novel method for tissue fixation, including subsequent paraffin-embedding and sectioning, that allows the complete pathological analysi... J Olert,KH Wiedorn,T Goldmann,... - 《Pathology Research & Practice》 被引量: 262发表: 2001年 Antigen Retrieval in Immunohistochemistry The...
The formation of tissue-engineered skin was evaluated using tissue sectioning methods. In addition, the suitability of the proposed hydrogel scaffolds for use in skin substitutes was evaluated in vivo. Access through your organization Check access to the full text by signing in through your ...
摘要: INTRODUCTIONThis protocol describes a method for embedding tissues in paraffin blocks for sectioning. Paraffin sections require extensive fixation and processing steps, but provide superior morphology compared with other sectioning methods.DOI: 10.1101/pdb.prot4989 被引量: 36 ...