The 3D positions were determined prior to histologic sectioning using a recently introduced 3D-microscopic approach. Individual histologic sections of villi were classified by their stromal structures and inter rater variability of these histologic assessments were determined.Inter rater variability was high ...
Science of Tissue Processing Fundamentals of Fixation and Tissue Processing When to Use a Vibrating Microtome for Tissue Sectioning Effects of Fixation and Tissue Processing on Immunocytochemistry Connective Tissue Rhondalyn Bomkamp RN, BSN, MBA Connective tissue is one of the body's four tissue type...
The tissues were fixed by formalin no longer than 48 hours, and then processed to be ready for sectioning. Two serial tissuesectionswith 5 µm thickness are mounted on a SuperFrost Plus glass slide. TissueArray.com histology paraffin tissue section is perfect fo fast detection of genes and ...
thus accurate classification and mapping of cell types needs to consider and integrate these distinct yet related cellular properties1,2. Single-cell RNA-sequencing (scRNA-seq) has enabled systematic classification at the transcriptomic level3,4,5, capturing major cell types with known...
E. Sullivan for tissue sectioning, E. Mackey for tissue sectioning and mouse colony management, N. Flytzanis and N. Goeden for their contributions towards histology, imaging, data analysis and manuscript preparation, P. Anguiano for administrative assistance, and the entire Gradinaru group for discu...
Because the macroscope does not perform optical sectioning, the transcranial fluorescence signal reflects fluorescence integrated vertically over the cortical volume, allowing collection of signals from across the cortex despite its curvature. To assign spatial localization of neural signals and register ...
Cell types in organoids matured in vitro to a stable “developed” state at a rate similar to human retina development in vivo. Transcriptomes of organoid cell types converged toward the transcriptomes of adult peripheral retinal cell types. Expression of disease-associated genes was cell-type-...
Samples were washed with PBS and embedded in OCT for 100-μm thick sectioning. Sections were blocked (PBS, 5% BSA, 1% Tween20) for 12 hours at 4°C, incubated with primary antibodies for 2 days at 4°C, then incubated with secondary antibodies for 2 days at 4°C. The following ...
(2) During sample preparation, a minority of samples were damaged from human error, such as cryosectioning severely orthogonal to the intended plane of sectioning, and excluded from further analysis. (3) Microscopy images of retinal or optic nerve sections were excluded if sectioning was orthogonal...
Crystal cell inclusions do not survive sectioning for EM, leaving a clear, rhomboid hole in the section. The inset shows a DIC image of a crystal (bright rhomboid inclusion) in a cell in a living Trichoplax; nuclear staining (cyan, arrow) shows a flattened nucleus. Scale bars represent 2 ...