paleontological techniquesskeletal histologyAndrea CuccuDepartamento de Ciencias de la Tierra Instituto Universitario de Investigación en Ciencias Ambientales de Aragón (IUCA), Universidad de Zaragoza Zaragoza SpainTeresa CalderónDepartamento de Ciencias de la Tierra Instituto Universitario de Investigación en ...
From patient to pathologist, preparing tissue specimens for histological examination requires care, skill and sound procedures. This guide provides practical advice on best-practice techniques and simple ways to avoid common errors. Tips for better tissue processing and embedding are highlighted in this ...
Current techniques including organizing, punching, and inserting tissues is time consuming and involving costly equipments.This Arraymold is the simple tool but great answer for this current technique for laboratories to perform TMAs with less time consuming, excellent results. With this Arraymold, you...
Despite widespread adoption of tissue clearing techniques in recent years, poor access to suitable light-sheet fluorescence microscopes remains a major obstacle for biomedical end-users. Here, we present descSPIM (desktop-equipped SPIM forcleared specimens), a low-cost ($20,000–50,000), low-ex...
Despite of the progress of numerous tissue clearing techniques, signal aberration, and intensity reduction in the deep region still remain to be major challenges in whole organ 3-D imaging. Serial block face imaging combining tissue clearing and sectioning technique should be a promising direction in...
We have developed techniques for glycol methacrylate embedding and sectioning of brain tissue blocks on a standard histology laboratory microtome. In addition, we have modified routine diagnostic and investigational neurohistological stains for use in glycol methacrylate embedded brain sections, including ...
In this study, we aim to establish 3D visualization protocol by combining neural tracing and tissue clearing techniques. We tested the potential of this protocol on tracing the neural circuit involved in Parkinson's disease dementia (PDD) by using both chemical-based and viral-based neural tracers...
The early histologists realized soon that apart from tissue sectioning appropriate preparation steps were needed for microscopical work with animal and human organs. The development of so-called histological microtechniques included stabilization of cell structures by chemicals such as solvents like ethanol...
AsbestosOrgan Culture TechniquesHistocytochemistryWe have recently developed culture techniques to allow the long-term maintenance of adult peripheral lung tissue in vitro from a variety of mammalian species including hamster, rat, bovine, and human. The technique involves perfusion of the major airways ...
which is not only labor-intensive and time-consuming but also error-prone. Recent advances in tissue clearing techniques allow visualization of cellular structures and neural networks inside of unsectioned whole tissues or the entire body. However, currently available protocols require long process time...