THP1-Dual™ Cells NF-κB-SEAP and IRF-Lucia luciferase reporter monocytes Catalog code: thpd-nfis https://www.invivogen.com/thp1-dual For research use only Version 23E29-MM PRODUCT INFORMATION Contents and Storage • 3-7 x 106 of THP1-Dual™ cells in a cryovial or shipping...
2-4 mM L-glutamine, 10% (v/v) heat‑inactivated fetal bovine serum (FBS; 30 min at 56 ºC), 50 U/ml penicillin, 50 mg/ml streptomycin, 100 mg/ml NormocinTMNotes:- The use of NormocinTM together with Pen-Strep is required to keep the cells free of microbial contaminants. Co...
Various treatments were then added to the cells and incubated overnight. After 18 to 20 h, the media/treatment was removed and luciferase activities were determined using Promega's Dual Luciferase Assay (Promega; Madison, MI). Viral infection Human SCD1 coding sequence was amplified by PCR ...
After treatments, cells seeded in glass-bottom cell culture dishes were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 followed by blocking with 3% bovine serum albumin (BSA). Then, the cells were immunolabelled with specific primary antibodies (TFEB, LC3 and Lamp2 at 1...
THP1-Dual™ hTLR9 Cells TLR9-overexpressing-NF-κB-SEAP & IRF-Lucia luciferase reporter monocytes Catalog code: thpd-htlr9 https://www.invivogen.com/thp1-dual-htlr9 For research use only Version 23A09-MM PRODUCT INFORMATION Contents • 3-7 x 106 THP1-Dual™ hTLR9 cells in a ...
We recently demonstrated that human macrophages derived from THP-1 monocytic cells, which lack TP53, are highly resistant to radiation-induced apoptosis compared with undifferentiated THP-1 cells. However, the mechanisms by which THP-1 cells acquire radioresistance during differentiation remain unknown. ...
Mycobacterium tuberculosisH37Rv;Ascaris lumbricoidesexcretory-secretory proteins;Jurkat cells;THP-1 cells;human tuberculosis and helminth co-infection;cytokine gene transcription levels 1. Introduction Tuberculosis (TB) infection is caused byMycobacterium tuberculosis(Mtb), a significant global health challenge an...