CELL PREPARATION 1. Centrifuge cells at 150 x g (RCF) for 10 minutes or at 300 x g (RCF) for 5 minutes. 2. Remove supernatant and resuspend THP1-Dual™ cells at 5 x 105 cells/ml in freshly prepared test medium. DETECTION OF TLR STIMULATION 1. Add 20 µl of sample...
THP1-Dual™ hTLR9 Cells TLR9-overexpressing-NF-κB-SEAP & IRF-Lucia luciferase reporter monocytes Catalog code: thpd-htlr9 https://www.invivogen.com/thp1-dual-htlr9 For research use only Version 23A09-MM PRODUCT INFORMATION Contents • 3-7 x 106 THP1-Dual™ hTLR9 cells in a ...
Cells were checked under a light microscope for the evidence of differentiation. 3.2.2. Co-Cultivation of Dihydrochalcones and THP1 Cells After 24 h rest, cells were incubated with final concentration of 100 µM of each dihydrochalcone (PZ and PT) as the concentration giving the most ...