Methods: We adapted our "Infection-on-a-Chip" microfluidic device to incorporate macrophages within the collagen extracellular matrix, allowing for the study of interactions between human neutrophils and macrop
加g口凇 serogroupIcterohaemollrhagiaeser0Vaur L越s慨n LajbeforeoraRerinfectionofhuIn锄THP-lmonocytelinewereextractedusingTritonX—ll4method锄d we佗detectedbyWestemblotusingthec0盯esponding锄tibodies.TheOMPsextr:acts、vereseparatedbytwo-dimensionalgelelectr.ophor℃sis(2一DGE)andthendetectedusingsilVer...
IL-1β in supernatant of THP-1-derived macrophages increased significantly at 1 hour after HCMV infection, was continuously rising at 3 hours and 6 hours subsequently, and reached at peak at 12 hours and maintained till 48 hours. The protein expression of IL-1β in HCMV infected group was...
However, the expression of beclin1 and LC3 transformation decreased obviously compared to the RAPA group at 24 h and 48 h after infection. For Heat-HCMV group, the expression of beclin1 increased significantly at 1 h after infection and was higher than that in the mock control group. LC3 ...
Rab18 facilitates dengue virus infection by targeting fatty acid synthase to sites of viral replication[J]. J Virol, 2014, 88(12):6793-6804. 18. Feldmann A, Bekbulat F, Huesmann H, et al. The RAB GTPase RAB1...
选择 24孔板铺板, 材料和方法 每个孔细胞量为 1.5×10/孔,24h后换液,再培养 一、 主要材料与试剂 24h,并于镜下观察铺板成功后,将培养液吸出,PBS 人 THP一1细胞来源于南京军 区南京总医院呼 清洗 2遍 ,将配好 的不 同感染复数 (multiplicityof 吸内科实验室;胰酶:碧云天公司;1640细胞培养基 infection,...
We detail steps for virus production, THP-1 cell infection and clone selection, fixing the suspension cells to the glass slide for immunostaining, and subsequent confocal microscopy. This protocol can be applied to other suspension cells. For complete details on the use and execution of this ...
MOImultiplicityofinfection感染复数 MODSmultipleorgansdisfunctionsyndrome 多功能脏器功能障碍 综合征 NALPneutrophilicalkalinephosphatase 嗜中性白细胞碱性磷 酸酶 NAIPneuronalapoptosisinhibitoryprotein神经元凋亡抑制蛋白 NLRP3 NACHT,LRRandPYD domains-containingprotein3 ...
In vitro expansion of human gastric epithelial stem cells and their responses to bacterial infection[J]. Gastroenterology, 2015, 148(1): 126-36.e6. [20] Misra R, Rajic M, Sathiyamoorthy K, et al. Ultrasound and microbubbles (USMB) potentiated doxorubicin penetration and distribution in 3D ...
影响及分子机制。方法采用结核分枝杆菌H37Rv株感染THP-1细胞，RT-PCR和Western blot检测CCL2 的表达。在THP-1细胞中转染CCL2siRNA,采用H37Rv感染下调CCL2的THP-1细胞，实验分为Mock组、H37Rv组、H37Rv+si-NC组和H37Rv+si-CCL2组，采用RT-PCR和Western blot分析CCL2的表达，菌落形成 单位计数分析H37Rv胞内存活率‘...