Industrial Production of Therapeutic Proteins: Cell Lines, Cell Culture, and PurificationThe biotechnology and pharmaceutical industries have seen a recent surge in the development of biological drug products manufactured from engineered mammalian cell lines. Since the hugely successful launch of human ...
Many of these disadvantages, especially the lack of post-translational modifications, can be overcome by employing large-scale animal cell cultures for the production of proteins of pharmaceutical interest. However, due to the long generation time and the requirement for rich culture media, the use ...
therapeutic proteins have emerged as a promising treatment option for various diseases, particularly cancer, cardiovascular disease, diabetes, and others. The formulation of protein-based therapies is a major area of research, however, a few factors still hinder the...
Cell culture media supplementation of bioflavonoids for the targeted reduction of acidic species charge variants on recombinant therapeutic proteins 来自 Wiley 喜欢 0 阅读量: 165 摘要: Charge variants in recombinant proteins are an important series of protein modifications, whose potential role on protein...
Lipoprotein particles that consist of dietary triglycerides, phospholipids, cholesterol and proteins produced in the enterocytes that transport lipids in other parts of the body. Thrombocytopenia A condition characterized by abnormally low levels of blood platelets that may result in bleeding problems. Lipod...
Understanding the factors that influence the therapeutic protein quality in rCHO cell culture will facilitate the development of large-scale, high-yield fed-batch culture processes for the production of high-quality therapeutic proteins. 展开
(LPS) stimulates non-classical inflammasomes directly. Guanylate binding proteins (GBPs), which is induced by interferon (IFN-γ), serve as the responder of cytosolic LPS and provide a platform for the recruitment and activation of caspase-4 [12]. Then GSDMD is cleaved and oligomerized on the...
Genetic engineering tools have given us the ability to “cut” and “paste” various genes that code for human proteins, and transfer them into the genome of bacteria, insect, or mammalian cells and grow them in culture so that they can express (produce) the desired human protein. Because ...
EVs contain a wide spectrum of biomolecules including proteins, lipids, nucleic acids (DNAs, RNAs, small RNAs). Together with the notion that EVs are a natural player of cell-cell interaction in multicellular organisms, studies have focused on strengthening their specific function [53]. For examp...
(for functionality in target cells) of mRNA and RNA-binding proteins is important. The fact that we could not detect strong luminescence without a C/Dboxin the 3′-UTR also indicates that the nluc delivery is indeed mediated by mRNA transfer into the exosomes and then the cytosol of target...