These reagents, as well as anti-puromycin antibodies, have played a pivotal role in advancing our understanding of the regulation and dysregulation of protein synthesis in normal and pathological processes, including immune response and neurological function. This manuscript reviews the current state of ...
- 《Journal of Food Science》 被引量: 17发表: 2003年 Effect of Puromycin on Cholinesterase Activity of Embryonic Chick Intestine in Organ Culture INTESTINE, SMALLPHARMACOLOGYPUROMYCINTISSUE CULTURETHE acetylcholinesterase (AChE) activity of a number of chick embryo tissues cultured in vitro can be ....
Firefly luciferase-green fluorescent protein (GFP) lentiviral particles (PLV-10172-50, Cellomics Technology) were used to transduce HT-29 cells (HT-29-Luc) at 1 × 108 TU per ml per followed by puromycin selection. Cell lines (purchased from ATTC) were frequently checked to be mycoplasma...
Puromycin (0.5 µg/ml) was used as a cell death-inducing positive control. (e) Cell cycle analysis of HT-29 cells after 24 h treatment with 5% (v/v) F-IEX and vehicle (same as in a). (f) Bar-chart quantifying result from panel e. Data presented as mean +/- SD from n ...
metaphase cells were collected. Double FISH with FISH probe against the Tet operator and against theMYCFISH probe was performed on the metaphase spread. PC3 cells with TetO repeats were infected with lentivirus containing F9-TetR-EGFP-IRES-PuroR; 2 d after infection, puromycin was added to ...
China) was used to enhance viral transfection efficiency in both cases. Following 48 h of lentiviral transfection, the medium was replaced with fresh medium containing 2 μg/mL puromycin (P8230, Solarbio) for one week to select stably transfected cells. For transient knockdown of ANXA2, cells ...
Protein degradation experiments were carried out in the presence of puromycin (50 μg/ml), an inhibitor of protein synthesis (30). At the indicated time points, HEK293 cells were harvested and subjected to Western blot analysis. Protein concentrations of the whole cell lysates were determined ...
1 µg.mL–1 of puromycin was used to select infected cells. After limit dilution, viable single clones were amplified and tested by immunoblotting to control for CEP131 negative expression, and CEP131 knockout was confirmed by genomic DNA sequencing after PCR amplification of CRISPR mutation...
After transfection for 72 h, the viral supernatant was collected, filtered and used for the transduction of breast cancer cells in the presence of 8 μg/ml polybrene (Merck Millipore). Stable cells were selected by 1 μg/ml puromycin (InvivoGen). Cell proliferation assay Cells were ...
After 48 h, medium containing the lentivirus was replaced and infected cells were selected by addition of puromycin (2 μg ml−1) or blasticidin (10 µg ml−1). Western blot analysis Detection of proteins by western blot analysis was achieved using standard protocols with ...