The resulting cell line was characterized for Sim1 expression by in situ hybridization, for glutamatergic marker expression by immunocytochemistry and quantitative real time polymerase chain reaction (qRT-PCR), and for functional maturation by electrophysiology. Puromycin selection significantly enriched the ...
cells is possible with puromycin, it is more commonly and effectively used for mammalian cell selection. It is active against gram-positive microorganisms, less active against acid-fast bacilli and more weakly active against gram-negative microorganisms.?The growth of bacteria, protozoa, algae and ...
Puromycin (CAS 58-58-2) is a selection antibiotic for mammalian, insect, and bacterial cells, available as a sterile, cell culture-tested solution.
(2) pBSpacand pBSpacΔP, which lack the SV40 small-t intron and thepBR322toxic sequences23; the first member of each class contains the original prokaryotic promoter of thepacgene. All these recombinants have been used for the selection of transformed mammalian cells by their growth in ...
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Now, puromycin hydrochloride is particularly useful for the selection of cell types harboring plasmids carrying puromycin resistance genes. Puromycinresistant cells express pac gene, which encodes an N-acetyl puromycin transferase. The pac gene can be mobilized on a plasmid and used to transfect a ...
antibiotic required to kill your host cell line. Puromycin quickly kills eukaryotic cells that do not contain the pac gene. Dying cells detach from the plates, allowing easy and early identification of transformant clones. Suggested working conditions for selection in some mammalian cells are listed...
Now, puromycin hydrochloride is particularly useful for the selection of cell types harboring plasmids carrying puromycin resistance genes. Puromycinresistant cells express pac gene, which encodes an N-acetyl puromycin transferase. The pac gene can be mobilized on a plasmid and used to transfect a ...
• Eukaryotic and bacterial selection See recommendations for working concentrations for selection antibiotics. Learn more about the use of antibiotics and antimycotics in cell culture, and review guidelines for decontaminating cultures. 规格 Validated Application: Eukaryotic Selection⁄Stable Cell Line ...
Puromycin's main application is in cell biology as a selective antibiotic agent in cell culture systems for selecting mammalian cell lines, which have been transformed by vectors that express puromycin-N-acetyl-transferase (PAC). Puromycin’s longstanding application as a gene selection antibiotic has...