bacteriaare resistant due to the low permeability of the antibiotic. For more than 30years, puromycin has been widely used as a basic tool for studying protein synthesis. Now, puromycin hydrochloride is particularly useful for the selection of cell types harboring plasmids carrying puromycin ...
cells is possible with puromycin, it is more commonly and effectively used for mammalian cell selection. It is active against gram-positive microorganisms, less active against acid-fast bacilli and more weakly active against gram-negative microorganisms.?The growth of bacteria, protozoa, algae and ...
Puromycin (CAS 58-58-2) is a selection antibiotic for mammalian, insect, and bacterial cells, available as a sterile, cell culture-tested solution.
Now, puromycin hydrochloride is particularly useful for the selection of cell types harboring plasmids carrying puromycin resistance genes. Puromycinresistant cells express pac gene, which encodes an N-acetyl puromycin transferase. The pac gene can be mobilized on a plasmid and used to transfect a ...
antibiotic required to kill your host cell line. Puromycin quickly kills eukaryotic cells that do not contain the pac gene. Dying cells detach from the plates, allowing easy and early identification of transformant clones. Suggested working conditions for selection in some mammalian cells are listed...
Linnell ER, Lerner CP, Johnson Ka, Leach Ca, Ulrich TR, et al. (2001) Transgenic mice for the preparation of puromycin-resistant primary embryonic fibroblast feeder layers for embryonic stem cell selection. Mammalian Genome 12: 169-171.
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Now, puromycin hydrochloride is particularly useful for the selection of cell types harboring plasmids carrying puromycin resistance genes. Puromycinresistant cells express pac gene, which encodes an N-acetyl puromycin transferase. The pac gene can be mobilized on a plasmid and used to transfect a ...
• Eukaryotic and bacterial selection See recommendations for working concentrations for selection antibiotics. Learn more about the use of antibiotics and antimycotics in cell culture, and review guidelines for decontaminating cultures. 规格 Validated Application: Eukaryotic Selection⁄Stable Cell Line ...
At different concentrations of puromycin and tetracycline respectively in the cell culture mediums, the growth of bovine kidney cells (MDBK) showed that the optimal puromycin resistant selection concentration was 3 μg/mL and tet. 通过分别利用不同浓度的嘌呤霉素和四环素来确定最佳筛选浓度和最佳调控浓...