What is the selection concentration of Puromycin in mammalian cells? The working concentration of Puromycin in mammalian cell lines varies between 1-10 μg/ml. In an initial experiment we recommend to determine the optimal concentration of puromycin required to kill your host cell line. Please note...
Puromycin’s?longstanding application as a gene selection antibiotic, has a powerful new role in Genome editing utilizing the CRISPR/Cas9 system. Purity Data is for ?a concentration of 10 mg/ml. ? Additional Information Synonyms Stylomycin, P638 Product # P-1033-SOL CAS # 58-58-2 Chemical ...
Puromycin is widely used in cell biology as a selection antibiotic agent in mammalian cell culture systems. The recommended working concentration ranges from 0.2–5.0 µg/mL, although it can be toxic to eukaryotic cells at concentrations as low as 1 µg/mL. Gibco® Puromycin Dihydrochloride...
(2) pBSpacand pBSpacΔP, which lack the SV40 small-t intron and thepBR322toxic sequences23; the first member of each class contains the original prokaryotic promoter of thepacgene. All these recombinants have been used for the selection of transformed mammalian cells by their growth in ...
At different concentrations of puromycin and tetracycline respectively in the cell culture mediums, the growth of bovine kidney cells (MDBK) showed that the optimal puromycin resistant selection concentration was 3 μg/mL and tet. 通过分别利用不同浓度的嘌呤霉素和四环素来确定最佳筛选浓度和最佳调控浓...
SpecificationsProduct concentration:10 mg/mlCAS number: 58-58-2 Quality Control:Each lot is thoroughly tested to ensure the absence oflot-to-lot variation.Purity:≥ 98% (HPLC)Endotoxin level:< 5 EU/mgPhysicochemical characterization:pH, appearanceCell-culture tested:potency validated inpuromycin-sens...
Suggested working conditions for selection in some mammalian cells are listed below. Cell line Medium Puromycin conc References B16 (Mouse melanocytes)RPMI1-2 μg/ml1, 2 HEK293 (Human embryonic kidney cells)DMEM0.5-10 μg/ml3-5 HeLa (Human uterine cells)DMEM1-10 μg/ml5, 6 ...
resistance is conferred by the expression of thepacgene.Puromycin is widely used in cell biology as a selection antibiotic agent in mammalian cell culture systems. The recommended working concentration ranges from 0.2–5.0 µg/mL, although it can be toxic to eukaryotic cells at concentrations as...
proteininacell-freepreparationfromratliver.Itwas postulatedthattheinhibitionwasduetothefactthat no 14 C-Leucinewastransferredfromsoluble(or transfer)ribonucleicacid- 14 C-Leucinetomicrosomal protein. 5 Aworkingconcentrationof50mg/mlofPuromycinhas beenusedforcompleteinhibitionofproteinsynthesis ...
Puromycin is widely used in cell biology as a selection antibiotic agent in mammalian cell culture systems. The recommended working concentration ranges from 0.2–5.0 µg/mL, although it can be toxic to eukaryotic cells at concentrations as low as 1 µg/mL. Gibco® Puromycin Dihydrochloride...