What is the selection concentration of Puromycin in mammalian cells? The working concentration of Puromycin in mammalian cell lines varies between 1-10 μg/ml. In an initial experiment we recommend to determine the optimal concentration of puromycin required to kill your host cell line. Please note...
Puromycin is widely used in cell biology as a selection antibiotic agent in mammalian cell culture systems. The recommended working concentration ranges from 0.2–5.0 µg/mL, although it can be toxic to eukaryotic cells at concentrations as low as 1 µg/mL. Gibco® Puromycin Dihydrochloride...
Puromycin’s?longstanding application as a gene selection antibiotic, has a powerful new role in Genome editing utilizing the CRISPR/Cas9 system. Purity Data is for ?a concentration of 10 mg/ml. ? Additional Information SynonymsStylomycin, P638 ...
(2) pBSpacand pBSpacΔP, which lack the SV40 small-t intron and thepBR322toxic sequences23; the first member of each class contains the original prokaryotic promoter of thepacgene. All these recombinants have been used for the selection of transformed mammalian cells by their growth in ...
Puromycin is widely used in cell biology as a selection antibiotic agent in mammalian cell culture systems. The recommended working concentration ranges from 0.2–5.0 μg/mL, although it can be toxic to eukaryotic cells at concentrations as low as 1 μg/mL. Gibco™ Puromycin Dihydrochloride is...
Product concentration: 10 mg/ml CAS number: 58-58-2 Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation. Purity:≥ 98% (HPLC) Endotoxin level: < 5 EU/mg Physicochemical characterization: pH, appearance Cell-culture tested: potency validated in puromy...
At different concentrations of puromycin and tetracycline respectively in the cell culture mediums, the growth of bovine kidney cells (MDBK) showed that the optimal puromycin resistant selection concentration was 3 μg/mL and tet. 通过分别利用不同浓度的嘌呤霉素和四环素来确定最佳筛选浓度和最佳调控浓...
Specifications Product concentration: 10 mg/mlCAS number: 58-58-2 Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.Purity:≥ 98% (HPLC)Endotoxin level: < 5 EU/mgPhysicochemical characterization: pH, appearanceCell-culture tested: potency validated in ...
Aβ-lactamase gene for plasmid selection and propagation in transformed prokaryotic cells in the presence of ampicillin An I-SceI homing restriction endonuclease site for linearization of constructs prior to transfection into mammalian cells, which favors integration into the target cell genome via the ...
Like DHFR, GS is an amplifiable marker6; that said, there is a question as to whether increasing selection stringency via increasing MSX concentration contributes to increased productivity via gene amplification5,7. A key (and ongoing) feature of the original GS selection system was the use of...