A diagram comparing the workflows used for the standard DNA extraction/purification approach and the direct PCR approach.doi:10.1371/JOURNAL.PONE.0044563.G001E. GilbertoB. JessicaF. NoahJames Grace lymiePLOS ONE
By this model, we achieved aged-like microglia in non-aged brains and confirmed that aged-like microglia per se contribute to cognitive decline. Collectively, our work provides a comprehensive resource for decoding the aging process of microglia, shedding light on how microglia maintain brain ...
The basic protocol for performing an IP is diagrammed below, where the order (sequence) of steps can be done in two different ways. Diagram of immunoprecipitation (IP) using either pre-immobilized or free antibodies. Each step involve...
The following diagram shows the protocol flow of the CHARRA attestation process. .---. .---. | Attester | | Verifier | '---' '---' | | | <--- requestAttestation(nonce, keyID, pcrSelection) | | tpmQuote(nonce, pcrSelection) | | => evidence | | | evidence ---> | | | ...
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RT-PCR analysis showed that IL-4 content in knockout mice was significantly increased after Schistosoma japonicum infection, yet the increase was less than that in infected wild-type mice (P < 0.05). PKCλ/ι deficiency led to reduced expression of fibrosis-related factors, including TGF-...
CRC development may take decades to transform into the final form of cancer. Many factors could modify the pace of this process, including diet, alcohol, and tobacco consumption, as well as contaminants present in water and food [5, 6]. Recently, the gut microbiota (GM) has also been ...
This process can induce depression-like behaviors in recipient animals, demonstrating a critical role of the gut microbiome in depression onset [13]. However, most of the studies on the issue of depression-associated of microbiota to date have focused on the effects of the “health or depression...
Ageing-associated functional decline of organs and increased risk for age-related chronic pathologies is driven in part by the accumulation of senescent cells, which develop the senescence-associated secretory phenotype (SASP). Here we show that procyani