Learn about the steps of mitosis, including what occurs in the cell at each stage and the main differences between mitosis and meiosis. Related to this QuestionDescribe the phases and overall purpose of interphase. What is G_0? Describe the events that occur during interphase. Explain the...
Describe the process of translation and transcription in the cell. Describe the steps of transcription and translation in a eukaryotic cell. What is the difference between the process of translation and transcription? What are the differences between the processes of translation and transcription? ...
We describe the steps for barcoding TFs, converting human fibroblasts to pancreatic ductal-like cells using defined TFs, and preparing library for FI-snMultiome-seq analysis. For complete details on the use and execution of this protocol, please refer to Fei et al.1 Graphical abstract Download:...
What are the role of DNA polymerase and RNA polymerase? Identify the three steps in polymerase chain reaction (PCR) and describe the function of each step. Identify which of the following enzymes has the following function: Relax the supercoiling of DNA and helps in unwinding. a. DNA polymera...
(SMCs) have been recently identified and establish that pre-mRNA splicing represents a target for therapy. We describe herein the identification of BPN-15477, a SMC that restores correct splicing ofELP1exon 20. Using transcriptome sequencing from treated fibroblast cells and a machine learning ...
Here, we describe the use of Cas13d-mediated RNA knockdown screens to identify breast cancer-associated lncRNAs that modulate proliferation in normal breast and breast cancer cells. Cas13d has previously been used to screen circular RNAs (circRNAs), with crRNAs designed to target their back-splic...
The ongoing pandemic caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), necessitates strategies to identify prophylactic and therapeutic drug candidates for rapid clinical deployment. Here, we describe a screening pipeline
Here, we present a protocol to obtain and isolate temperature-sensitive (TS) SARS-CoV-2 strains via 5-fluorouracile-induced mutagenesis. We describe steps for the induction of mutations in the wild-type virus and selection of TS clones. We then show how to identify the mutations associated ...
Numerous small satellite networks that we describe implicate several biologically important genes as possible drivers of TNBC that were not previously linked to this disease. We also noted important driver-network differ- ences between patient cancers and cell lines. This suggests that some network ...
Single-cell Hi-C (scHi-C) analysis has been increasingly used to map chromatin architecture in diverse tissue contexts, but computational tools to define chromatin loops at high resolution from scHi-C data are still lacking. Here, we describe Single-Nucleus Analysis Pipeline for Hi-C (SnapHiC)...