Here, we present a protocol to screen human donors for the CCR5delta32 (螖32) polymorphism, which confers resistance to HIV infection, and identify the sex of the human donor used to produce HIS mice. We describe
Describe the steps of transcription and translation in a eukaryotic cell. What is the difference between the process of translation and transcription? What are the differences between the processes of translation and transcription? (a) What are the three stages of transcription? (b) Describe th...
By integrating various identification approaches, the number of initially annotated SEPs in this bacterium increases from 27 to 329, with a quarter of them predicted to possess antimicrobial potential. Herein, we describe a methodology complementary to Ribo-Seq and mass spectroscopy that can identify ...
Distinguish between clonal anergy and clonal expansion. What factor determines which of these two processes will take place? Describe the steps of oocyte production. Cnidocytes are used for what two processes? The 5' capping process creates what type of linkage?
Theobroma cacao L. cultivar Matina 1-6 belongs to the most cultivated cacao type. The availability of its genome sequence and methods for identifying genes responsible for important cacao traits will aid cacao researchers and breeders. We describe the se
In this report, we describe a new approach to isolate the sequences of neoantigen-specific TCRs. We further demonstrated that these TCRs could recognize neoantigens presented by autologous APCs in four examples. We found that the early T cell activation marker IFN-γ mRNA was a valuable marker...
Taken together, we propose a hypothesis to describe the mechanism of male-induced female reproductive development (Supplementary Fig. 13). Male worms develop a strong neuromuscular function together with some extracellular matrix components, such as the gynecophoral canal protein58, to sense and grasp...
Here, we describe the use of Cas13d-mediated RNA knockdown screens to identify breast cancer-associated lncRNAs that modulate proliferation in normal breast and breast cancer cells. Cas13d has previously been used to screen circular RNAs (circRNAs), with crRNAs designed to target their back-splic...
Explain. b) Name and describe the steps of DNA separation? Explain. c) How is DNA analyzed? Explain. How do bases pair in complementary strands of DNA? Explain. Why is knowing the structure of DNA important? How does DNA polymerase interact with DNA? How does...
Describe a bacterial genome. Identify the two steps in gene expression. Explain and discuss what occurs in each step, including where the steps occur and the nucleic acids involved. How do nucleotides provide nucleic acids with specific codes? HINT: Think about...