Why is RT-PCR used? What is the difference between it and normal PCR? What is PCR and why is it useful? Explain the Ct value in PCR. What does PCR stand for and what is its general purpose? Discuss the process of PCR. What does it do?
Polymerase chain reaction (PCR) is a laboratory procedure that can create replicas of DNA. Explore the three steps of this revolutionary process: denaturation, annealing, and extension. Related to this Question How does PCR work? Can PCR work with a single-stranded DNA template?
The following figure shows the process of PCEP session maintenance. The PCC and PCE periodically send Keepalive messages to each other in order to maintain the PCEP session until it is closed. If one end does not receive the Keepalive message from the other end within a specified period, the...
Once the instrument is started during the process, the amplification procedure cannot stop to add new samples, and the next batch of sample amplification must be completed after this batch is completed. This is one of the re...
Applied Biosystems TaqMan assays consist of target-specific primers and probes, optimized for real-time PCR. The mechanisms are described here.
Also, some of your customized Windows settings may be lost through this process.How to do a repair installation of WindowsA repair installation will restore the current Windows installation to the version of the installation DVD. It also requires the installation of all updates that...
The amplification of DNA is achieved through a process called polymerase chain reaction (PCR). PCR works by repeatedly heating and cooling DNA. When DNA is heated, it separates into two single strands. Next, an enzyme called “Taq polymerase” is introduced to the sample. This enzyme ...
How does the RT-PCR tell you if a gene is expressed in a particular cell type? Once mRNA is created through transcription, it is often processed. Explain how mRNA can be processed. Include the names of important enzymes or structures. ...
Is there a work-around, or is this change necessary for the overall success of the project? Does this change require an increase in funding? Will this delay the project end date? Even though this change may have a negative impact on this project, does it result in significant b...
How does one analyze DNA by the use of DNA fingerprinting? How is DNA Used to Identify a Crime Suspect? What are it's other uses? Briefly describe the DNA fingerprinting application. How is a DNA fingerprinting test interpreted? A DNA sweep or dragnet is a ...